rev:   October 19. 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human INTERLEUKIN-5

-Interleukin-5 (IL-5) is a potent T cell derived factor which stimulates the growth of certain B- cells and the differentiation of eosinophils. This recombinant Human Il-5 is a 26 kDa protein containing 232 amino acid residues.

Catalog#: RDI-205

Package Size: 10 micrograms $231.00    $219.00/pack 2+

                        Mini-Pack: cat#RDI-205p $94.00/2ug
                       -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder with no additives.


Source: E. Coli

Purity: >98% by SDS-Page analysis . Endotoxin level is less than 0.1ng per ug (1EU/ug) of IL-5.

Reconstitution: The lyophilized IL-5 should be reconstituted in water to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4 DEG C, mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for 1 week or long term at - 20 DEG C for future use. For most in-vitro applications, IL-5 exerts its biological activity in the range of 0.1 to 10.0 ng/ml.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted IL-5 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 as determined by the dose-dependent stimulation of human TF-1 cells was less than 0.15ng/ml, which corresponds to a specific activity of >6 X 10 E06 units/mg..

AA Sequence:

MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF

QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE

RRRVNQFLDY LQEFLGVMNT EWIIES


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Bulk available upon request.


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