rev:    November 8, 2004

Return (RDI HOME PAGE)

Return (human cytokine index)


Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human INTERLEUKIN-4

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. This recombinant human IL-4 is a 14.9 kDa globular protein containing 129 amino acid residues

Catalog#: RDI-204  

                               $235.00/package   $219.00/package 2 or more

                            Mini-Pack: cat#RDI-204p $94.00/2ug

                            -inquire for custom sizes/bulk packaging


Package Size: 10 micrograms

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis and HPLC analysis. Endotoxin level is less than 0.1 ng per ug (1EU/ug) of IL-4.

Reconstitution: The lyophilized IL-4 should be reconstituted in water to a maximum concentration of 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, rotate, mix well. If desired,centrifuge for 1-2 minutes at 500-1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at  4 DEG C for up to 1 week or at -20 DEG C for future use. For most in-vitro applications, IL-4 exerts its biological activity in the range of 0.1 to 10.0 ng/ml.

Note: For material that will not be used for coating or labelling (or other applications requiring carrier free material), we recommend adding 0.1% protease free BSA/HSA or media to any dilution buffers. Maintain stock concentrations of >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated below at -20 DGE C. Reconstituted IL-4 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 as determined by the dose- dependent stimulation of human TF-1 cells is <0.2ng/ml, corresponding to a specific activity of >5 X 10 e06 units/mg.

AA Sequence:

MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA

TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC

PVKEANQSTL ENFLERLKTI MREKYSKCSS

Precautions: For In vitro research Use Only. Not for use in or on   humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Return (RDI HOME PAGE)

Return (human cytokine index)


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

Ordering terms

all rights reserved by any/all copyright holders?