rev:    November 7, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human INTERLEUKIN-2

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. This recombinant human IL-2 is a 15.5 kDa protein, containing 134 amino acid residues including one intrachain disulfide bond.

Catalog#: RDI-202

Package Size: 50 micrograms $235.00   $219.00/package 2 or more

                     see below for bulk material cat#RDI-202-209X

                       Mini-Pack: cat#RDI-202p $94.00/10ug
                   
Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >97% by SDS-Page analysis and HPLC analysis. Endotoxin level is less than 0.1 ng per ug (1EU/ug) of IL-2.

Reconstitution: The lyophilized IL-2 should be reconstituted in 100mM acetic acid to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4 DEG C, Mix Well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. For most in-vitro applications, IL-2 exerts its biological activity in the range of 0.1 to 1.0 ng/ml.

Note:Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted IL-2 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The Ed50 as determined by the dose dependent stimulation of murine CTLL-2 cells is <0.1ng/ml. The specific activity is greater than 1 X 107 units/mg as determined by the CTLL assay.

AA Sequence:

MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM

LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH

LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN

RWITFAQSII STLT


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Recombinant Human Interleukin-2   (rHuIL-2) BULK

cat# RDI-202-209X   $1125.00/mg   $1000.00/mg 3+

       RDI-202-209X5  $375.00/100ug

       RDI-202-209S    $250.00/50ug

Bulk quotes on request

Description: Recombinant Human Il-2 is produced in E.Coli is a single, non-glycosylated mutein (variant form), of human IL-2 polypeptide chain containing 134 amino acids and having a molecular mass of 5517Dalton. This recombinant human IL-2 has a Ser substitute for cysteine at position 126. The rHuIL-2 is purified by proprietary chromatographic techniques.

Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Source: E.Coli

Endotoxin: Less than 0.1 ng/µg (IEU/µg) of IL-2.

Formulation: The protein was lyophilized after extensive dialysis against 0.17mg sodium phosphate monobasic and 0.89mg dibasic phosphate buffer pH7.5.

Purity: Greater than 98.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Anion-exchange FPLC.

(c) Analysis by reducing and non-reducing SDS-PAGE

Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be

Met-Ala-Pro-Thr-Ser.

-

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Solubility: It is recommended to reconstitute the lyophilized rHuIl-2 in sterile 20mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rHuIL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuIL-2 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze-thaw cycles.

Biological Activity: IL-2 is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine CTLL-2 cells in the presence is <0.065ng/ml, corresponding to a Specific Activity of 16.9 million IU/mg

Protein content: Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.614 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of IL-2 as a Reference Standard.

For In vitro research Use Only. Not for use in or on humans or animals .  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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