rev:   October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human INTERLEUKIN-13 Analog

-Interleukin-13 is an immunoregulatory protein secreted predominantly by activated Th2 cells. It plays a key mediator in the pathogenesis of allergic inflammation and contributes to airway hyper-reactivity. A variant of IL-13 shows enhanced functional activity compared with the wild type IL-13(Q130). This genetic variant, termed IL-13 analog, is a mature 114 amino acid protein with a substitution of Q for R at position 112.

Catalog#: RDI-2013A

Package Size: 10 micrograms $231.00/package   $219.00/pack 2+

                      Mini-Pack# cat#RDI-2013Ap $94.00/2ug

                -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder from 10mM Sodium Citrate pH4.0 with no additives. Endotoxin level is less than 0.1ng/ug (1EU/ug).

Source: E. Coli

Purity: >98% by SDS-Page analysis

Reconstitution: At first use, tighten cap and gently tap on solid surface or quick spin 30 seconds 1000rpm to concentrate any material in bottom of vial. Carefully open top. Lyophilized IL-13 analog should be reconstituted in sterile water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, mix well. If desired, centrifuge 1 minute 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted IL-13A should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: This IL-13 analog shows a two fold increase, relative to wild type IL-13, in bioactivity as measured by the in vitro dose dependent activation of STAT6 and IL-13 dependent gene induction in transfected A201.1 cells. This analog has also been shown to exhibit increased in vivo activity compared to wild type IL-13, as measured by the induction of airway hyper-responsiveness.

AA Sequence :

MSPGPVPPST ALRELIEELV NITQNQKAPL CNGSMVWSIN LTAGMYCAAL

ESLINVSGCS AIEKTQRMLS GFCPHKVSAG QFSSLHVRDT KIEVAQFVKD

LLLHLKKLFR EGQFN

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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