rev:     November 7, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human INTERLEUKIN-6 RECEPTOR

IL-6 mediates its biological effects through the type I IL-6 receptor system that consists of two chains, IL-6R alpha and gp130. The IL-6R alpha chain is the binding component specific to IL-6; while the gp130 only transmits signals of IL-6 when bound to IL-6R alpha. The gp130 also can transmit signals from LIF, OSM, CNTF, IL-11 and CT-1 in conjunction with other receptor subunits. The low-affinity binding site for IL-6 is composed of IL-6R alpha alone. IL-6R alpha is expressed in a wide range of cells including T cells, fibroblasts and macrophages. Soluble IL-6R alpha which consists of only the extracellular domain of the IL-6R alpha chain, acts as an agonist of IL-6 activity at low concentrations. Recombinant human sIL-6R is a 37.6 kDa protein consisting of the extracellular domain of the IL-6R alpha chain (338 amino acid residues).

Catalog#: RDI-2006R

Package Size: 20 micrograms   $231.00/package    $219.00/package 2 or more

                          Mini-Pack:cat#RDI-2006p $94.00/5ug

                       -inquire for custom sizes/bulk packaging


Supplied: The sterile filtered solution was lyophilized from 1 x PBS, pH 7.2.
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Source: HEK 293 cells

Purity: >98% by SDS-Page analysis and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/UG) of IL-6R.

Reconstitution: We recommend a quick spin followed by reconstitution in 1 x PBS, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Allow to set at least 10 minutes. Rotate and/or vortex to mix. Centrifuge for 1-2 minutes at 1000 rpm to concentrate solution in bottom of vial.This solution can then be diluted into other aqueous buffers and stored at 4 DEGC for 1 week or stored at -20 DEG C for futures use.

Note::If this material is not to be used for coating or labeling or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted IL-6R should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 as determined by it's ability to intensify the IL-6 induced growth inhibition of murine M1 cells is < 10.0 ng/ml, in the presence of 20 ng/ml of rhIL-6.

sequence:

LAPRRCPAQE VARGVLTSLP GDSVTLTCPG VEPEDNATVH

WVLRKPAAGS HPSRWAGMGR RLLLRSVQLH ASGNYSCYRA

GRPAGTVHLL VDVPPEEPQL SCFRKSPLSN VCEWGPRSTP

SLTTKAVLLV RKFQNSPAED FQEPCQYSQE SQKFSCQLAV

PEGDSSFYIV SMCVASSVGS KFSKTQTFQG CGILQPAPPA

NITVTAVARN PRWLSVTWQD PHSWNSSFYR LRFELRYRAE

RSKTFTTWMV KDLQHHCVIH DAWSGLRHVV QLRAQEEFGQ

GEWSEWSPEA MGTPWTESRS PPAENEVSTP MQALTTNKDD

DNILFRDSAN ATSLPVQD

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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