rev:      October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human INTERLEUKIN-4 RECEPTOR  ALPHA

Human IL-4 exerts its biological effects via signaling through its receptor system, IL-4R. There are two types of the IL-4 receptor complexes, the type I receptor consisting of the IL-4R alpha and the common gamma chain, yc and the type II receptor consisting of IL-4R alpha and the IL-13R alpha. The IL-4R alpha chain contains an extracellular domain of 207 amino acids, a transmembrane domain of 24 amino acids, and an intracellular domain of 569 amino acids. The secreted extracellular domain, sIL-4R alpha, also called CD124 is capable of blocking IL-4 activities. This receptor plays a major role in the regulation of the differentiation of naive CD4+ T cells and class switching to IgG1 and IgE. This recombinant human sIL-4R alpha is a 23.7 kDa protein containing 207 amino acid residues consisting of only the extracellular domain of the IL-4R alpha.


Catalog#: RDI-2004R

Package Size: 15 micrograms $231.00/package  $219.00/package 2 or more

                       Mini-Pack:cat#RDI-2004p $94.00/3ug


Supplied: The sterile filtered solution was lyophilized from 1 x PBS, pH 7.2.

Source: A 293 cells

Purity: Greater than 97% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).

Reconstitution: We recommend a quick spin followed by reconstitution in 1 x PBS, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Allow to set at least 10 minutes. Rotate and/or vortex to mix. Centrifuge for 1-2 minutes at 1000 rpm to concentrate solution in bottom of vial.This solution can then be diluted into other aqueous buffers and stored at 4 DEGC or 1 week or stored at -20 DEG C for futures use.

Note::If this material is not to be used for coating or labeling or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted IL-4R should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 as determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells is < 5.0 ng/ml (in the presence of 0.5 ng/ml IL-4), corresponding to a specific activity of > 2 x 105 units/mg.

AA Sequence :

MKVLQEPTCV SDYMSISTCE WKMNGPTNCS TELRLLYQLV

FLLSEAHTCI PENNGGAGCV CHLLMDDVVS ADNYTLDLWA

GQQLLWKGSF KPSEHVKPRA PGNLTVHTNV SDTLLLTWSN

PYPPDNYLYN HLTYAVNIWS ENDPADFRIY NVTYLEPSLR

IAASTLKSGI SYRARVRAWA QCYNTTWSEW SPSTKWHNSY

REPFEQH

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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