rev:    January 16, 2005

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:Recombinant GDF-3

GDF-3 is a member of the TGF-ß superfamily of growth and differentiation factors, and is highly homologous to GDF-9. Unlike most TGF-ß family members, GDF-3 and GDF-9 are not disulfide-linked dimers. GDF-3 is expressed in adult bone marrow, spleen, thymus, and adipose tissue. The expression of GDF-3 is upregulated in high-fat-fed wild-type FABP4/aP2 null mice and was associated with obesity, but not with the relatedhyperglycemia/hyperinsulinemia which characterizes Type 2 diabetes. This recombinant human GDF-3 is a 26.0 kDa non-disulfide-linked homodimer containing two 114 amino acid polypeptide chains.

Catalog#: RDI-1222

Package Size: 20 micrograms $231.00 $219.00/pack 2+

                     Mini-Pack: cat#RDI-1022P $94.00/5ug  

Supplied: Lyophilized powder from with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of protein

Reconstitution: Lyophilized material should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. -Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or HSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted GDF-3 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Activity Data not available at this time.

AA Sequence :

AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA

NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDE CGCG

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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