rev:   October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


recombinant Human CTGFL/WISP-2

CTGFL/WISP-2 is a 28.6 kDa protein that belongs to the CCN family of cysteine rich regulatory proteins. Members of this family stimulate mitosis, adhesion, apoptosis, extracellular matrix production, growth arrest, and migration of multiple cell types. The protein is expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart. In addition to promoting adhesion of osteoblasts, CTGFL/WISP-2 inhibits osteocalcin production, as well as binding of fibrinogen to integrin receptors. This recombinant human CTGFL/WISP-2 is a 24.8 kDa protein of 228 amino acid residues.

Catalog#: RDI-1216

Package Size: 20 micrograms $231.00   $219.00/pk 2 or more

                        MINI-PACK cat#RDI-1216p $94.00/5ug

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >95% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of CTGFL.

Reconstitution: The lyophilized protein is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water to a max concentration of 0.1-1mg/ml. NOTE: Do not raise to neutral pH. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or at - 20 DEG C for future use.

Note:If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted human CTGFL should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is between 10-20 ng/ml in the presence of 15 ng/ml of human IGF-II.

AA Sequence:

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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