rev:    October 19, 2004

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HUMAN Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:Human Follistatin

Follistatin belongs to a group of structurally-diverse diffusible proteins that binds to TGF-beta ligands and inhibit their activity by hindering access to signaling receptors. This naturally occurring antagonist binds to Activin, BMP-2,-4,-6,-7, Myostatin, GDF-11, and TGF-beta1. Follistatin is expressed in the pituitary, ovaries, decidual cells of the endometrium, and in some other tissues. This recombinant human Follistatin is a 31.5 kDa protein containing 288 amino acids including 36 cysteine residues.

Catalog#: RDI-1213

Package Size: 20 micrograms   $231.00    $219.00/pack 2+

                       Mini-Pack:cat#RDI-1213P $94.00/5ug

                     -inquire for custom sizes/bulk packaging

Supplied: The sterile filtered solution was lyophilized from 10mM sodium Phosphate pH7.5 + 75mM NaCl with no additives

Source: E. coli

Purity: >95% by SDS-Page and HPLC analysis Endotoxin <1EU/ug

Reconstitution: The lyophilized human Follistatin should be reconstituted in sterile DI water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4-8 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. . This solution can be diluted into other buffered solutions and stored at 4 DEG C for up to 1 week or stored at -20 DEG C for future use.

Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to further dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted material should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

AA Sequence:

GNCWLRQAKN GRCQVLYKTE LSKEECCSTG RLSTSWTEED VNDNTLFKWM

IFNGGAPNCI PCKETCENVD CGPGKKCRMN KKNKPRCVCA PDCSNITWKG

PVCGLDGKTY RNECALLKAR CKEQPELEVQ YQGRCKKTCR DVFCPGSSTC

VVDQTNNAYC VTCNRICPEP ASSEQYLCGN DGVTYSSACH LRKATCLLGR

SIGLAYEGKC IKAKSCEDIQ CTGGKKCLWD FKVGRGRCSL CDELCPDSKS

DEPVCASDNA TYASECAMKE AACSSGVLLE VKHSGSCN

Reactivity: Determined by its ability to neutralize Activin A   inhibitory effect of murine MPC-11 cells. The expected ED50 us 0.1-0.4ug/ml in the presence of 7.5ng/ml Activin A.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent  infringements that might result with the use of or derivation of this product.



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