rev:    October 19, 2004

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HUMAN Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:Human GDF-11 is a myostatin-homologous protein that acts as an inhibitor of nerve tissue growth. GDF-11 has been shown to suppress neurogenesis through a myostatin-like pathway, which involves arrest of progenitor cell-cycle in the G1 phase. Similarities between myostatin and GDF-11, which are 90% identical in their amino acid sequence, suggests that the regulatory mechanisms responsible for maintaining proper tissue size during neural and muscular development might be the same. This recombinant human GDF-11 is a 25.0 kDa disulfide-linked homodimer containing two 109 amino polypeptide chains.

Catalog#: RDI-1211

Package Size: 20 micrograms $231.00   $219.00/pack 2+

                       Mini-Pack:cat#RDI-1211P $94.00/5ug

                     -inquire for custom sizes/bulk packaging

Supplied: The sterile filtered solution was lyophilized with no additives

Source: E. coli

Purity: >98% by SDS-Page and HPLC analysis Endotoxin <1EU/ug

Reconstitution: The lyophilized human GDF-11 should be reconstituted in sterile DI water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4-8 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial. . This solution can be diluted into other buffered solutions and stored at 4 DEG C for up to 1 week or stored at -20 DEG C for future use.

Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted material should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 was determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 0.08-0.10 µg/ml.

AA Sequence:


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent  infringements that might result with the use of or derivation of this product.



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