rev:    June 2, 2005

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  recombinant Human TRANSFORMING GROWTH FACTOR-A

-TGF-a is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-a induces transformation anchorage independence in cultured cells. Human, murine and rat TGF-a are cross-species reactive. This Recombinant human TGF-a is a 50 amino acid polypeptide (5.5 kDa) which shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds.

Catalog#: RDI-116a

Package Size: 100 micrograms $231.00/vial      $219.00/vial 2+

                        Mini-Pack: cat#RDI-116ap $94.00/20ug

                        inquire for bulk

Supplied: Lyophilized protein with no additives

Source: E. Coli

Purity: >98% by SDS-Page analysis and N-terminal sequence analysis. Endotoxin less than 0.1ng per ug of TGF-A.

Reconstitution: The lyophilized material is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in 10mm acetic acid to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions containing carrier protein or medium and stored at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted TGF-A should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb-c 3T3 cells is <0.7ng/ml corresponding to a specific activity of >1.4 X 10 E06 units/mg.

AA Sequence:

VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG

ARCEHADLLA


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements with use/derivation of this product.


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phone (978) 371-6446

fax     (978)  371-2266

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