rev:    October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human Platelet Derived Growth Factor-AA

-Platelet derived growth factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AA is a 28.5 kDa A chain homodimer containing 250 amino acid residues.


Catalog#: RDI-113a


Package Size: 10 micrograms $231.00    $219.00/pack 2+

                   Mini-PACK: cat#RDI-113ap $94.00/2ug
                  -inquire for custom sizes/bulk packaging

Supplied: Lyophilized protein with no additives

Source: E. Coli

Purity: >98% by SDS-Page analysis and N-terminal sequence analysis. Endotoxin less than 0.1ng per ug of PDGF-AA.

Reconstitution: The lyophilized material is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in 10mM acetic acid to a concentration of 0.1-1mg/ml. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at - 20 DEG C for future use. For most in- vitro applications, PDGF-AA exerts its biological activity in the range of 0.2 to 10.0 ng/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted PDGF-AA should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb-c 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of >1 million unts per milligram.

AA Sequence:

MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE

VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK

EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR

KRLKPT

IPrecautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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