rev:   October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Insulin Like Growth Factor II

-Insulin Like Growth Factor-II (IGF-II) is a polypeptide growth factor which stimulates the proliferation of a wide range of cell types . Human IGF-II is a 7.5 kDa protein containing 67 amino acid residues.


Catalog#: RDI-112

Package Size: 50 micrograms $231.00     $219.00/package 2 +

                    Larger sizes on request

                     Mini-Pack cat#RDI-112p $94.00/10ug


Supplied: Lyophilized protein with no additives

Source: E. Coli

Purity: >98% by SDS-Page analysis and HPLC analysis. Endotoxin less than 0.1ng per ug of IGF-II.

Reconstitution: The lyophilized material is soluble in water and most aqueous buffers. We recommend reconstituting the lyophilized powder in sterile DI water to a maximum concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and mix well. If desired, centrifuge at 1000 rpm for 1-2 minutes to concentrate material in vial. This solution can be diluted into other buffered solutions and stored at 4 DEG C for up to one week or stored at - 20DEG C for future use. For most in-vitro applications, IGF-II exerts its biological activity in the range of 0.5 to 25.0 ng/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but is best stored desiccated at -20 DGE C. Reconstituted IGF-II should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

AA Sequence:

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR

GIVEECCFRS CDLALLETYC ATPAKSE

Reactivity: The Ed50 was determined by a cell proliferation assay using FDC-P1 cells is <2.0ng/ml, corresponding to a specific activity of >500K units per mg.

Old comparative test: The ED50 as determined by competitive binding against plasma derived IGF-II to human placental membranes is <1ng/ml, corresponding of a specific activity of >1 million units/milligram.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for   diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

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phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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