rev:    October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


DATA SHEET:recombinant Human EMAP-II

Endothelial-Monocyte Activating Polypeptide-II (EMAP-II) is a novel cytokine that is an antiangiogenic factor in tumor vascular development, and strongly inhibits tumor growth. Recombinant human EMAP-II is an 18.3 kDa protein containing 166 amino acid residues.

Catalog#: RDI-1038

Package Size: 20 micrograms $231.00    $219.00/pack 2+

                      Mini-Pack: cat#RDI-1038p $94.00/5ug  

                                  -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder from with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of protein

Reconstitution: Lyophilized EMAP-II should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. -Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or HSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted EMAP-II should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by the apoptotic effect on MCF-7 cells (human breast carcinoma cells) using a concentration of 20-40 ng/ml.

AA Sequence :

SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP

RTVVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM

VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP

KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM

SNSGIK
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


anti-EMAP-II

cat#RDI-EMAP2abGP $212.00/50ug

cat#RDI-EMAP2abGP1 $300.00/100ug

Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hEMAP-II.

Anti-EMAP-II specific antibody was purified by affinity chromatography employing

immobilized hEMAP-II matrix.

anti-hEMAP-II:Biotin conjugated

-cat#RDI-EMAP2abGPB $212.00/25ug

cat#RDI-EMAP2abGPB1 $300.00/50ug

Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hEMAP-II

. Anti-hEMAP-2 specific antibody was purified by affinity chromatography and then

biotinylated.


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phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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