rev:   October 19, 2004   BACKORDER early 2005

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


DATA SHEET:RECOMBINANT Human TRANSFORMING GROWTH FACTOR-Beta 3

-Transforming Growth Factor-Beta 3 (TGF-B3) is a member of a superfamily of homologous, disulfide-linked, homodimeric proteins that regulate the proliferation and differentiation of normal and transformed cells. This recombinant Human TGF-B3 is a 25.0 kDA protein with each subunit containing 112 amino acid residues.

Catalog#: RDI-1036

Package Size: 5 micrograms $235.00   $219.00/vial 2+  Bulk quotes on request

Supplied: Lyophilized protein from sodium citrate, pH3.5 buffer with no additives

Source: A293 cells

Purity: >98% by SDS-Page analysis and HPLC analysis. Endotoxin less than 0.1ng per ug of TGF-B3.

Reconstitution: Human TGF-B3 is very soluble in buffers at pH's below 4. However, at neutral pH's TGF-B3 tends to stick to surfaces of tubes, pipets, etc. This TGF-B3, when reconstituted in water to a concentration of 20ug/ml (ie, 2ug/100ul) will yield a solution of 5mM citrate buffer pH 3.5 that can be stored at room temp or 4 DEG C for one months. Dilution of this stock solution should take into account the stickiness of the protein. It is recommended that further dilutions be made into PBS containing 2mg/ml albumin and stored in working aliquots at -20 DEG C.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted stock TGF-B3 (20ug/ml in citrate buffer) can be stored at room temp or 4 DEG C for at least 1 month. More dilute solutions of TGF-B3 in PBS/2mg/ml albumin should be stored at -20 DEG C.AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 was determined by TGF-B3 ability to inhibit the mouse IL-4 dependent proliferation of mouse HT-2 cells is less than 0.05ng/ml, corresponding to a specific activity of >2 X 10 E08 units per milligram.

AA Sequence:

ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY

ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP

QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


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Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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