rev:   October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


recombinant Human Fibroblast Growth Factor-5

-Fibroblast Growth Factor-5 (FGF-5) , is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. This recombinant human FGF-5 is a 27.6 kDa protein containing 252 amino acid residues.

Catalog#: RDI-1034

Package Size: 50 micrograms $231.00   $219.00/pack 2 or more

                       MINI-PACK cat#RDI-1034p $94.00/10ug

                  -inquire for custom sizes/bulk packaging

Supplied: sterile filtered and the the protein was lyophilized from 2X PBS pH7.2( no additives)

Source: E. Coli

Purity: >95% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/ug) of FGF-5.

Reconstitution: The lyophilized FGF-5 should be reconstituted in 1X PBS pH7.2 to a concentration of 1ug/ul (1mg/ml). Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or stored at - 20 DEG C for future use. For most in-vitro applications, human FGF-5 exerts its biological activity in the concentration range of 0.1 to 20.0ng/ml.


Note:If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration >50ug/ml.

Storage: The lyophilized powder is stable at room temperature but it is best stored desiccated below 0 Deg C. Reconstituted human FGF-5 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES. EACh FREEZE THAW CYCLE WILL DIMINISH ACTIVITY.

Reactivity: The biological activity was determined by the does- dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors yielding an ED50 <1ng/ml, corresponding to a specific activity of >1 X 10 E06 (1 million) units/mg.

AA Sequence :

MAWAHGEKRL APKGQPGPAA TDRNPIGSSS RQSSSSAMSS SSASSSPAAS

LGSQGSGLEQ SSFQWSPSGR RTGSLYCRVG IGFHLQIYPD GKVNGSHEAN

MLSVLEIFAV SQGIVGIRGV FSNKFLAMSK KGKLHASAKF TDDCKFRERF

QENSYNTYAS AIHRTEKTGR EWYVALNKRG KAKRGCSPRV KPQHISTHFL

PRFKQSEQPE LSFTVTVPEK KNPPSPIKSK IPLSAPRKNT NSVKYRLKFR

FG

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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