rev:    Ocotber 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human Stem Cell Growth Factor-BETA

Human Stem Cell Growth Factor BETA (SCGF-beta) formerly known as SCGF is a newly discovered hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. Human SCGF-beta is a non-glycosylated 29 kDa species specific cytokine that can support growth of primitive hematopoietic cells, and in combination of EPO or GM-SCF, it promotes proliferation of erythroid and myeloid progenitors, respectively.

Catalog#: RDI-1022B

Package Size: 10 micrograms $231.00/package    $219.00/package 2+

                     Mini-PacK:cat#RDI-1022BP $94.00/2ug

                             -inquire for custom sizes/bulk packaging

Supplied: Lyophilized protein from sterile filtered solution of 0.25x PBS + 1mM DTT, with no additives.

Source: E. Coli

Purity: >95% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human SCGF-beta.

Reconstitution: The lyophilized human SCGF-beta is soluble in water and most aqueous buffers. For optimum dilution, the lyophilized powder should be reconstituted to a maximum concentration of 1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and mix gently by rotation or inversion. If desired can centrifuge for 1 minute at 1000rpm to concentrate material in vial .This solution can be diluted into other buffered solutions containing carrier protein or medium containing serum. or stored at -20 DEG C for future use. For most in vitro applications, SCGF-beta exerts its biological activity in the concentration range of 0.2 to 20.0 ng/ml

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder, though stable at room temperature, is best stored dessicated below 0 DEG C. Reconstituted material should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

AA Sequence

MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL

PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG

PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV

ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC

LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN

CVAQASDDGS WWDHDCQRRL YYVCEFPF

Reactivity: The biological activity is determined by its inhibitory effect on the   proliferation of TF-1 cells, previously grown in media containing GM- CSF. The Ed50 for this effect was observed at 7ng/ml.


Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


RDI Division of Fitzgerald Industries Intl

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Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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