rev:  October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human Stem Cell Growth Factor-Alpha

Human Stem Cell Growth Factor Alpha (SCGF-alpha) is a newly discovered hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. Human SCGF-alpha is a non-glycosylated 33.9 kDa species specific cytokine that can support growth of primitive hematopoietic cells, and in combination of EPO or GM-SCF, it promotes proliferation of erythroid and myeloid progenitors, respectively.

Catalog#: RDI-1022A

Package Size: 10 micrograms $231.00/package    $219.00/package 2+

   Mini-PacK:cat#RDI-1022AP $94.00/2ug

Supplied: Lyophilized protein from sterile filtered solution with no additives.

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of human SCGF-alpha.

Reconstitution: The lyophilized human SCGF-alpha is soluble in water and most aqueous buffers. For optimum dilution, the lyophilized powder should be reconstituted to a maximum concentration of 0.5mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and mix gently by rotation or inversion. If desired can centrifuge for 1 minute at 1000rpm to concentrate material in vial .This solution can be diluted into other buffered solutions containing carrier protein or medium containing serum. or stored at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder, though stable at room temperature, is best stored dessicated below 0 DEG C. Reconstituted material should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

Reactivity: The biological activity is determined by its inhibitory effect on the proliferation of TF-1 cells, previously grown in media containing GM- CSF. The Ed50 for this effect was observed at< 2.5ng/ml.

AA Sequence:

MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL

PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG

PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV

ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC

LKGLRLGHKC FLLSRDFEAQ AAAQARCTAR GGSLAQPADR

QQMEALTRYL RAALAPYNWP VWLGVHDRRA EGLYLFENGQ

RVSFFAWHRS PRPELGAQPS ASPHPLSPDQ PNGGTLENCV

AQASDDGSWW DHDCQRRLYY

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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