rev:     November 9, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


DATA SHEET:   RECOMBINANT Human TRANSFORMING GROWTH FACTOR-Beta 1

-The three mammalian isoforms of TGF-beta, TGF-beta1, beta2, beta3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and would healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-beta isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-beta1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors. Human TGF-beta 1 is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a single disulfide bond. *Manufactured using (BTI-Tn-5B1-4) cells under license from the Boyce Thompson Institute for Plant Research, Inc.

Catalog#: RDI-1021R

Package Size: 5 micrograms     $231.00    $219.00/vial 2+                     

Supplied: Lyophilized protein from sodium citrate, pH3.5 buffer with no additives

Source: (BTI-Tn-5B1-4) High-5 insect cells

Purity: >98% by SDS-Page analysis and HPLC analysis. Endotoxin less than 0.1ng per ug of TGF-B1.

Reconstitution: Human TGF-B1 is very soluble in buffers at pH's below 4. However, at neutral pH's TGF-B1 tends to stick to surfaces of tubes, pipets, etc. This TGF-B1, when reconstituted in water to a concentration of 50ug/ml (ie, 5ug/100ul) will yield a solution of 1mM citrate buffer pH 3.5 that can be stored at room temp or 4 DEG C for one monhhs. Dilution of this stock solution should take into account the stickiness of the protein. It is recommended that further dilutions be made into PBS containing 2mg/ml albumin and stored in working aliquots at -20 DEG C.

Storage: The lyophilized powder is stable at room temperature for afew weeks but it is best stored desiccated at -20 DEG C. Reconstituted stock TGF-B1 (50ug/ml in citrate buffer) can be stored at room temp or 4 DEG C for at least 1 months. More dilute solutions of TGF-B1 in PBS/albumin should be stored at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 was determined by TGF-B1 ability to inhibit the mouse IL-4 dependent proliferation of mouse HT-2 cells is less than 0.05ng/ml, corresponding to a specific activity of >2 X 10 E07 (20million) units per milligram.

AA Sequence:

ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH

ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP

QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS

mfg using (BTI-Tn-5B1-4) cells under license from the Boyce Thompson Institute for Plant Research Inc


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


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