rev:      October 18, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


see below VEGF(165) & VEGF 121

see also Elisa kits RDI-DA066 RELIDA VEGF-A 96 wells  $750.00/96 wells

                              RDI-DA068  Human VEGF-A Elisa 96 wells  $750.00/96wells

                              RDI-DA070  Human VEGF-C Elisa 96 wells  $750.00/96 wells

                              RDI-DA072  Mouse VEGF-A Elisa 96 wells $750.00/96 wells

                              RDI-DA074  RAt VEGF-C elisa 96 wells $750.00/96 wells


recombinant Human Vascular Endothelial Cell Growth Factor (165)

Vascular Endothelial Cell Growth Factor (VEGF) is a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability. Human VEGF is a 38.2 kDa homodimeric protein consisting of two 165 amino acid polypeptide chains.


Catalog#: RDI-1020

Package Size: 10 micrograms $231.00   $219.00/pk 2 or more

                     MINI-PACK cat#RDI-1020p $94.00/2ug

                       -inquire for custom sizes/bulk packaging

Supplied: Lyophilized powder with no additives

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug of VEGF.

Reconstitution: The lyophilized VEGF is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water to a max concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or at - 20 DEG C for future use. For most in-vitro applications, human VEGF exerts its biological activity in the concentration range of 1.0 to 50.0 ng/ml.

Note:If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted human VEGF should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

AA Sequence:

MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY

PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ

HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC

KNTDSRCKAR QLELNERTCR CDKPRR

Reactivity: Determined by the dose dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-8.0 ng/ml

old comparative test: The biological activity was determined by its mitogenic activity on human dermal microvascular endothelial cells. Mitogenic activity was obtained in a concentration range of 1.0 to 100 ng/ml.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


cat#RDI-300-075  rec human VEGF 165 (insect cells)  $250.00/5ug

cat#RDI-300-076  rec human VEGF 165 (insect cells)  $500.00/20ug

Description: Human Vascular Endothelial Growth Factor VEGF165, a 23 kDa protein consisting of 165 amino acid residues, is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT -1), and VEGFR-2 (KDR). Consistent with the endothelial cell-specific action of VEGF165, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extravillous tropho-blasts. In addition to its action as a mitogen it is a potent vasular permeability factor (VPF) in vivo. VEGF165 is also a chemoattractant molecule for monocytes and endothelial cells. 5 different proteins are generated by diffential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abdundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is appa-rently a homo -dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bio-availability is very different. There is good evidence that heterodimeric molecules between the different isoforms also exists and that different cells and tissues express different VEGF isoforms. The other members of this in-creasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.

Source: Insect cells

Molecular Weight: 45 kDa

Specific activity: 1 x 10 6 units/mg

Purity: > 90%, by SDS-PAGE and visualised by silver stain

Endotoxin level: < 0.1 ng per ug of VEGF

Stabilizer: none

Buffer: none

Formulation: lyophilized

Biologically Activity: The ED50 for stimulation of 3 H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGF165 has been determined to be in the range of 1-2 ng/ml.

Reconstitution: The lyophilised VEGF165 is soluble in water and most aqueous buffers. The lyophilised VEGF165 should be reconstituted in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 µg/ml.

Stability: Lyophilised samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF165 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!

Usage: VEGF165 is offered for in vitro research use only-not for use in or on humans or animals. Not for use in diagnostics.

Literature: [Breier et al., Dev 114:521, 1992; Fiebig et al., Eur J Biochem 211:19, 1993; Flamme et al., Dev Biol 162:699, 1995; Kremer at al., Cancer Res 57:3852, 1997]

** please note : always centrifuge vials before opening **


DATA SHEET:recombinant Human Vascular Endothelial Cell Growth Factor121 (VEGF 121)

VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. VEGF signals through three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Due to its increased acidity, VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates. This recombinant human VEGF121 is a 28.4 kDa disulfide-linked homodimeric protein consisting of two 121 amino acid polypeptide chains.

Catalog#: RDI-1020A

Package Size: 10 micrograms $231.00  $219.00/pk 2 or more

                   MINI-PACK cat#RDI-1020Ap $94.00/2ug

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug of VEGF.

Reconstitution: The lyophilized VEGF121 is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water to a max concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4 DEG C for up to 1 week or at - 20 DEG C for future use.

Note:If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted human VEGF should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by the dose dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) usaing a concentration range of 0.2-0.4   ng/ml

AA Sequence:

APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS

CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN

KCECRPKKDR ARQENCDKPR R

Precautions: For In vitro research Use Only. Not for use   in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Recombinant Human VEGF121 (insect cells)

Cat#RDI-300-031   $312.00/5ug

Cat#RDI-300-032   $750.00/20ug

Description: Human Vascular Endothelial Growth Factor121 (VEGF121), a 18 kDa protein consisting of 121 amino acid residues is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF121 have been identified, VEGFR-1 (FLT -1), and VEGFR-2 (KDR). consistent with the endothelial cell-specific action of VEGF121, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells.  Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extravillous trophoblasts. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo, and a chemoattractant molecule for monocytes and endothelial cells. Five different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF121 is apparently a homo -dimer, but preparations of VEGF show some heterogeneity on SDS gels depending on the secretion of different forms and the varying degrees of glycosylation. All dimeric forms posses similar biological activities but the bioavailability is very different. There is good evidence that heterodimeric molecules between the different isoforms exist and that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D, -E and PlGF.

Source:                       Insect cells

Molecular Weight:     ~36 kDa

Specific activity:        5 x 10 5 units/mg

Purity:                         > 90%, by SDS-PAGE and visualised by silver stain

Endotoxin level:        < 0.1 ng per ug of VEGF

Stabilizer:                  none

Buffer:                        none

Formulation:               lyophilized

Biological Activity: The ED50 for stimulation of 3 H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGF121 has been determined to be in the range of 2-5 ng/ml.

Reconstitution: The lyophilised VEGF121 is soluble in water and most aqueous buffers. The lyophilised VEGF121 should be reconstituted in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 µg/ml.

Stability: Lyophilised samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF121 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!

Usage: VEGF121 is offered for in vitro research use only-not for use in or on humans or animals.. Not for drug use. Not for human use!

Literature: [Breier et al., Dev 114:521, 1992; Fiebig et al., Eur J Biochem 211:19, 1993; Flamme et al., Dev

Biol 162:699, 1995; Kremer at al., Cancer Res 57:3852, 1997]

** please note : always centrifuge vials before opening **


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