rev:    October 18, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:Recombinant Human IGF-BP3, also known as growth-hormone-dependant binding protein, is 30 kDa cysteine-rich secreted protein. It is the major IGF binding protein present in the plasma of human and animals and it is also found in a-granules of platelets. In addition to its ability to dulate the activity of IGF-I and IGF-2, IGF-BP3 exerts inhibitory effects on follicle stimulating hormone (FSH) activity. Decreased plasma levels of IGF-BP3 often results in dwarfism, whereas elevated levels of IGF-BP3 may lead to acromegaly. The expression of IGF-BP3 in fibroblasts is timulated by mitogenic growth factors such as Bombesin, Vasopressin, PDGF, and EGF. Recombinant human IGF-BP3 is a 28.8 kDa protein consisting of 264 amino acid residues.

Catalog#: RDI-1008

Package Size: 25 micrograms $231.00     $219.00/pack 2+

                         Mini-pack:cat#RDI-1004P $94.00/10ug

                       -inquire for custom sizes/bulk packaging

Supplied: The sterile filtered solution is lyophilized from   20 mM HCl with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) .

Reconstitution: The lyophilized protein is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water, to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4 DEG C. If desired, can centrifuge for 1 minute at 1000 rpm to concentrate material in vial. This solution can be diluted into water or other buffered solutions or stored at -20 DEG C for future use.

Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder, though stable at room temperature for a few weeks, is best stored dessicated at -20 DEG C. Reconstituted material should be stored in working aliquots at -20 DEG C. Avoid Frequent Freeze-THAW Cycles.

Biological Activity: The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 for this effect is <0.2 mg/ml in presence of 15 ng/ml of human IGF-II.

Precautions: For In vitro research Use Only. Not for use continued on back in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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