rev:  December 23, 2004

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Cytokines, Growth Factors & Chemokines: 

(Rat and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Recombinant Rat VEGF-C 152S

-see also rec rat VEGF-C-

Description: VEGF-C152S is a point mutant generated by the replacement of the second conserved Cys residue of the recombinant processed VEGF-C by a Ser residue. VEGF-C152S is analog to the human VEGF-C156S mutant and only active toward VEGFR-3/FLT-4 but, unlike wild type VEGF-C, is unable to bind to and to activate signalling through VEGFR-2/KDR. VEGF-C152S was inactive in the vascular permeability assay and did not increase migration of the capillary endothelial cells, indicating that these VEGF-like effects of VEGF-C require VEGFR-2 binding.

VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The rat VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults, VEGF-C is highly expressed in heart, placenta, ovary and small intestine. Recombinant rat VEGF-C, lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain, forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT-4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells, it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C is also a mitogen for vascular endothelial cells, it is much less potent than VEGF-A. The recombinant rat VEGF-C contains 129 amino acids residues and was fused to a His-tag (6x His) at the C-terminal end. VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.

Source:                         Insect cells

Molecular Weight:       36-48 kDa

Biological Activity:     5 x 10 E03 units/mg

Purity:                          > 90%, by SDS-PAGE and visualised by silver stain

Endotoxin level:           < 0.1 ng per mg of VEGF-C

Stabilizer:                     BSA

Buffer:                        none

Formulation:               lyophilized

Biological Activity: Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells (PAE/FLT-4 cells). The ED50 for this effect is typically 150-300 ng/ml.

Reconstitution: The lyophilised VEGF-C  152S is soluble in water and most aqueous buffers. The lyophilised VEGF-C should be reconstituted in water, then dilute dinto PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 µg/ml.

Stability: Lyophilised samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF-C should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!

Usage: VEGF-C is offered for research use. Not for drug use. Not for human use!

Catalogue number: RDI-R20-016      $250.00/5ug       $219.00/vial 5+

                               RDI-R20-017      $500.00/20ug

Range: 100-400 ng/ml

Literature: [Joukov et al., EMBO J 15:290, 1996; Olofsson et al., Curr Opin Biotech 10:528, 1999 ; Kukk et al., Development 122:3829, 1996] Kirkin et al., Eur J Biochem 268:5530, 2001]

** please note : always centrifuge vials before opening **

see also rat  VEGF-C


-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.

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RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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