rev:  October 16, 2003

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:  recombinant Human PIGF-2

Cat#RDI-300-019         $312.00/5ug vial      

Cat#RDI-300-020          $750.00/20ug vial    $688.00/vial 5+

Description: Human Placenta Growth Factor (PlGF) is a polypeptide growth factor and a member of the platelet-derived growth factor family but more related to vascular endothelial growth factor (VEGF). PlGF acts only as a weak mitogen for those cell types possessing receptors for binding (e.g. vascular endothelial cells). At least one high-affinity receptor for PlGF (FLT-1 or VEGF-R1) has been demonstrated in different primary cell types(e.g. human umbilical vein endothelial cells and monocytes). In addition to its action as a weak mitogen it is also a chemoattractant for monocytes and endothelial cells. Two different proteins are generated by differential splicing of the human PlGF gene: PlGF-1 (131 aa native chain) and PlGF-2 (152 aa native chain). Both mitogens are secretable proteins, but PlGF-2 can bind to heparin with high affinity. PlGF is apparently a homodimer, but preparations of PlGF show some heterogeneity on SDS gels depending of the varying degrees of glycosylation. All dimeric forms posses similar biologcal activities. If PlGF is angiogenic in vivo is not clear. However, heterodimers between VEGF and PlGF are mitogenic for endothelial cells and have strong angiogenic activity in vivo (e.g. in the CAM assay or in the cornea pocket assay). Different cells and tissues (e.g. placenta) express PlGF-1 and PlGF-2 at different rates. A very related protein of PlGF is VEGF with about 53% homology and VEGF-B with similar biological activities.

Source:                        Insect cells

Molecular Weight:       44 kDa

Specific activity:         1million (1 x 10 E06) units/mg

Purity:                          80 % (SDS-PAGE, HPLC)

Endotoxin level:           < 0.1 ng per ug of PIGF-2

Stabilizer:                     BSA (carrier free available on quotation)

Buffer:                           none

Formulation:                  lyophilized

Biological Activity: Measured by its ability to bínd to immobilized rh Flt-1/Fc chimera in a functional ELISA.

Reconstitution: The lyophilised PlGF-2 is supplied in lyophilized form with carrier-protein (BSA) and can be reconstituted with 0.1 M acetic acid or PBS. This solution can be diluted into other buffered solutions or stored frozen for future use.

Stability: The lyophilized human PIGF-2, though stable at room temperature, is best stored in working aliquots at -20°C to -70°C.

Usage: Human PIGF-2 is offered for research use. Not for drug use. Not for human use.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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