rev:   July 6, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


PLGF-1  see 3 forms below
Recombinant Human PIGF-1

cat#RDI-300015   $312.00/5ug

cat#RDI-300016   $750.00/20ug  $688.00/vial 5+

Description: Placenta growth factor (PlGF) is a polypeptide growth factor and a member of the platelet-derived growth factor family but more related to vascular endothelial growth factor (VEGF). PlGF-1 acts only as a very weak mitogen for some endothelial cell types and as a potent chemoattractant for monocytes. The physiological function in vivo is still controversy. In several reports it was shown not to be a potent mitogen for endotehlial cells and not angiogenic in vivo by using different assays. Very recently it was shown by one investigator, that PlGF-1 from cell culture supernatants was angiogenic in the CAM assay and in the rabbit cornea assay. At least one high-affinity receptor for PlGF (FLT-1 or VEGF-R1) has been demonstrated in different primary cell types (e.g. human umbilical vein endothelial cells and monocytes) but PlGF does not bind to KDR/flk-1. Two different proteins can be generated by differential splicing of the human PlGF gene: PlGF-1 (131 aa native chain) and PlGF-2 (152 aa native chain). Both mitogens are secretable proteins, but PlGF-2 can bind to heparin with high affinity. PlGF-1 is a homodimer, but preparations of PlGF show some heterogeneity on SDS gels depending of the varying degrees of glycosylation. All dimeric forms posses a similar biological profile. There is good evidence that heterodimeric molecules between VEGF and PlGF exists and that they are biological active. Different cells and tissues (e.g. placenta) express PlGF-1 and PlGF-2 at different rates. A very related protein of PlGF is VEGF with about 53% homologyand VEGF-B with similar biological activities.

Source: Insect cells

Molecular Weight: 38 kDa

Specific activity: 1 x 106 units/mg

Purity: 90 % (SDS-PAGE, HPLC)

Endotoxin level: < 0.1 ng per ug of PIGF-1

Stabilizer: none

Buffer: none

Formulation: lyophilized

Biological Activity: The ED50 for stimulation of 3H-thymidine incorporation by human umbilical vein endothelial cells and bovine aortic endothelial cells for PlGF has been determined to be in the range of 2 ng/ml for half-maximal stimulation and 5-20 ng/ml for maximal stimulation. It is chemotactic for endothelial cells and monocytes in the same range.

Reconstitution: The lyophilised PlGF-1 is supplied in lyophilized form with carrier-protein (MSA) and can be reconstituted with 0.1 M acetic acid or PBS. Recommend initially reconstituting with 0.1M acetic acid or DI water, let set 30 minutes, mix gently, then dilute further in PBS or buffer of choice . This solution can be diluted into other buffered solutions or stored frozen for future use.

Stability: The lyophilized human PIGF-1, though stable at room temperature, is best stored in working aliquots at -20°C to -70°C.

Usage: Human PIGF-1 is offered for research use. Not for drug use. Not for human use.

Catalogue number: RDI-300-016 Size: 20 µg

Lot number : 0109R01

Range: 0.1-10.0 ng/ml


DATA SHEET: PLGF-1 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PLGF-1 is expressed in placental tissues, colon and mammary carcinomas. It signals through the VEGFR-1/FLT1 receptor and stimulates endothelial cell proliferation and migration. This recombinant human PLGF-1 is a 29.7 kDa disulfide-linked homodimeric protein of two 131 amino acid polypeptide chains.

Catalog#: RDI-1006

Package Size: 25 micrograms $212.00 $200.00/pack 2+

                         Mini-Pack:cat#RDI-1006P $94.00/5ug

Supplied: The sterile filtered solution was lyophilized without carrier or additives

Source: E. Coli

Purity: >98% by SDS-Page gel and HPLC analysis Endotoxin <1EU/ug

Reconstitution: The lyophilized human PLGF-1 should be reconstituted in sterile DI water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4-8 DEG C, Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate material in vial.This solution can be diluted into other buffered solutions and stored at 4 DEG C for up to 1 week or stored at -20 DEG C for future use.

Note: If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1%-1% BSA to further dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted material should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: Determined by its ability to chemoattract human monocytes of using a concentration range of 1.0-10.0 ng/ml.

AA Sequence :

MLPAVPPQQW ALSAGNGSSE VEVVPFQEVW GRSYCRALER LVDVVSEYPS

EVEHMFSPSC VSLLRCTGCC GDENLHCVPV ETANVTMQLL KIRSGDRPSY

VELTFSQHVR CECRPLREKM KPERCGDAVP RR

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Recombinant Human PIGF-1-His Tag

Catalogue number: RDI-300-017 Size: 5µg   $312.00

cat#RDI-300-018  20ug   $750.00

Description: Placenta growth factor (PlGF) , a 19kda protein consisting of 131 amino acid residues and fused to a C-terminal His-tag (6x His), is produced as a homodimer. PLGF is a polypeptide growth factor and a member of the platelet-derived growth factor family but more related to vascular endothelial growth factor (VEGF). PlGF-1 acts only as a very weak mitogen for some endothelial cell types and as a potent chemoattractant for monocytes. The physiological function in vivo is still controversy. In several reports it was shown not to be a potent mitogen for endotehlial cells and not angiogenic in vivo by using different assays. Very recently it was shown by one investigator, that PlGF-1 from cell culture supernatants was angiogenic in the CAM assay and in the rabbit cornea assay. At least one high-affinity receptor for PlGF (FLT-1 or VEGF-R1) has been demonstrated in different primary cell types (e.g. human umbilical vein endothelial cells and monocytes) but PlGF does not bind to KDR/flk-1. Two different proteins can be generated by differential splicing of the human PlGF gene: PlGF-1 (131 aa native chain) and PlGF-2 (152 aa native chain). Both mitogens are secretable proteins, but PlGF-2 can bind to heparin with high affinity. PlGF-1 is a homodimer, but preparations of PlGF show some heterogeneity on SDS gels depending of the varying degrees of glycosylation. All dimeric forms posses a similar biological profile. There is good evidence that heterodimeric molecules between VEGF and PlGF exists and that they are biological active. Different cells and tissues (e.g. placenta) express PlGF-1 and PlGF-2 at different rates. A very related protein of PlGF is VEGF with about 53% homologyand VEGF-B with similar biological activities.

Source:                        Insect cells

Molecular Weight:      38 kDa

Specific activity:       1 x 10 E06 units/mg

Purity:                        90 % (SDS-PAGE, HPLC)

Endotoxin level:        < 0.1 ng per ug of PIGF-1

Stabilizer:                  BSA or HSA

Buffer:                       none

Formulation:              lyophilized

Biological Activity: Measured by its ability to bind to immobilized thFLT-1/Fc chimera in a functional Elisa

Reconstitution: The lyophilised PlGF-1 is supplied in lyophilized form with carrier-protein (HSA) and can be reconstituted with 0.1 M acetic acid or PBS. Recommend initially reconstituting with 0.1M acetic acid or DI water, let set 30 minutes, mix gently, then dilute further in PBS or buffer of choice . This solution can be diluted into other buffered solutions or stored frozen for future use.

Stability: The lyophilized human PIGF-1, though stable at room temperature, is best stored in working aliquots at -20°C to -70°C.

Usage: Human PIGF-1 is offered for research use. Not for drug use. Not for human use.

Catalogue number: RDI-300-017 Size: 5µg     Lot number : 0109R01

Range: 0.1-10.0 ng/ml     Centrifuge vial before opening


and recombinant human PLGF-2 cat#RDI-300-019


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