rev:  May 12, 2005

Return (RDI HOME PAGE)

Return (human cytokine index)


Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


Recombinant Human Interferon Beta 1A (Hu-IFN-B) (mammalian)

CAT# RDI-PB11410     $375.00/vial 100KU     $312.00/vial 5+

       #RDI-PB11410X    $625.00/1 million Units



Quantity Shipped: 1 vial of 1 x 100K units in 0.1 ml.

Concentration: 1 x 10(6) units/ml

Buffer:   10mM Acetic acid

Mr 23,000;  >95% purity, one N-glycosylation site

Source: cDNA obtained from human fibroblast mRNA expressed in CHO CELLS

Accession number: V00534

Specific Activity:  3.99 X 10 E08 untis/mg

Assay Used to Measure Bioactivity: Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37, 755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assays for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for human interferon alpha A (Hu-IFN-aA) provided by the National Institutes of Health [see Pestka, S.(1986)"Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. Unit of activity measured on bovine MDBK cells with vesicular stomatitis virus (VSV).

Shipping Temperature: Wet ice (DO NOT FREEZE)

Physical State of Product During Shipping: Liquid

Special Conditions/Comments: DO NOT FREEZE. After receipt, this product should be kept at 2-8 DEG C. Tighten cap, centrifuge 1 minute 1000rpm to concentrate the contents on  the bottom of the tube. Dilute only in buffers containing protein such as 0.1% bovine serum albumin (BSA).

-For In Vitro Research Use Only-


-For In Vitro Research Use OnlyPrecautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Return (RDI HOME PAGE)

Return (human cytokine index)

Ordering terms

RDI Div of Fitzgerald Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

all rights reserved by any/all copyright holders?