rev:  October 2, 2001

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Cytokines, Growth Factors & Chemokines:ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


KERATINOCYTE GROWTH FACTOR

(FGF-7)


Rabbit Anti-human Keratinocyte Growth Factor (anti-hKGF)   Antigen Affinity Purified Polyclonal Antibody

Catalog Number: RDI-KGFabrP      $212.00/50ug

                            RDI-KGFabrP1    $300.00/100ug

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hKGF. Anti-hKGF specific antibody was purified by affinity chromatography employing immobilized hKGF matrix.

Formulation: A sterile filtered antibody solution (1mg/ml) was lyophilized from 0.5X PBS pH 7.4.

Stability/ Reconstitution: The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20oC. When reconstituted in sterile water to a concentration of >0.2 mg/ml the antibody is stable for at least six weeks at 2-4oC. Add 200ul DI water (50ug/vial=250ug/ml stock).

Application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hKGF ( ng/ml), a concentration of ug/ml of this antibody is required.

ELISA: To detect hKGF by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hKGF.

Western Blot: To detect hKGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hKGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Usage: For Research Use Only. Not for use in diagnostic procedures.

also available Biotin conjugated:

cat#RDI-KGFabrPB     $212.00/25ug

cat#RDI-KGFabrPB1   $300.00/50ug

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


DATA SHEET: Mouse anti-KGF (Keratinocyte Growth Factor)

Catalog#: RDI-KGFabm

Batch#

Package Size: 100 micrograms $438.00/vial    $375.00/vial 3 or more

Supplied: as neat liquid (approx 200ul (0.5mg/ml) in 0.1M   Tris-HCL, pH 7.5)

antigen:purified human KGF (reverse phase HPLC purified)


Source: mouse

Isotype: IgM

Purity: IgM purified fraction using Protein G


Application -ELISA

-western blot (must use an IgM specific antibody with good sensitivity and specificity)

-All titers must be determined in assay

Storage: Store at -20 DEG C. Avoid frequent freeze thaw   cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

see also recombinant human KGF.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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