rev:   October 17, 2004

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Cytokines, Growth Factors & Chemokines:ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


INSULIN LIKE GROWTH FACTOR 2

(please also see our Insulin data Pack for a more complete lising of our insulin/ILGF1 & 2, & ILGFR antibodies).


Rabbit anti-human ILGF2(antigen aff pure, lyophilized, no carrier/nopreservative)

cat#RDI-ILGF2abrP      $250.00/50ug

cat#RDI-ILGF2abrP1   $500.00/100ug


DATA SHEET: Mouse anti-Rat/Human Insulin Like Growth Factor II

Catalog#: RDI-ILGF2abm   Price: $594/vial

Package Size: 500 micrograms (in 0.5ml sterile filtered PBS buffer,pH 7.4 no preservatives or carrier added)

Species: mouse IgG1 purified via ammonium sulphate precipitation and DEAE-cellulose chromatography.

Antigen: partially purified rat IGF-II

Specificity: specific for IGF-II of rat and human origin (kd=5.9 X 10E9); less than 10% cross with human IGF-I.

Activity: Neutralization: 100 micrograms/ml to inhibit the stimulatory effect of 100 nanograms IGF- II/ml on DNA synthesis of human fibroblasts and sulfation by chick cartilage.

              Western blot: 1 micrograms/ml to detect 10- 100ng IGF-II

               Immunocytochemistry: 0.1ug/ml-1ug/ml -on 10% formalin fixed tissue (streptavidin biotin detection), or approx 1-10ug/ml on acetone/methanol (1:1) fixed frozen sections. -Overnight 4 DEG C incubations -MUST TITER FOR EACH APPLICATION

ref: Endocrinology, 124:870-877, 1989

Storage: Upon receipt, store at -20 DEG C or below. -After reconstitution, aliquot and store at - 20 DEG C. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.


MOUSE MONOCLONAL ANTIBODY TO: HUMAN INSULIN-LIKE GROWTH FACTOR II

PRODUCT CODE    RDI-CBL82    $375.00/vial

                           

CLONE      W2H1

ISOTYPE   IgG1

IMMUNIZING ANTIGEN  Human anti-globulin conjugated to IGF-II

FUSION PARTNER   NS1 myeloma cell line

SOURCE  Mouse ascitic fluid

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY

The antibody recognises IGF-II, cross-reactivity with IGF-I <0.01% and insulin <0.01%.

APPLICATIONS

*Measurement of serum IGF-II levels

*Suitable for use in RIA, ELISA, IRMA or immuno-histochemistry (frozen sections only)

LOT SPECIFIC DATA

PRESENTATION The monoclonal is presented at a concentration of 0.1mg in 0.5ml phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE

For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.



RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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