rev:    March 11, 2005

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Cytokines, Growth Factors & Chemokines:ANTIBODIES  

(anti-Human and others as indicated)


RDI Division of Fitzgerald Industries Intl  offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


INSULIN LIKE GROWTH FACTOR I

(please request our Insulin data Pack for a more complete lising of our insulin/ILGF1 & 2, & ILGFR antibodies).


Rabbit anti-human ILGF1 (antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-ILGF1abrP     $250.00/50ug

cat#RDI-ILGF1abrP1   $375.00/100ug

GOAT anti-mouse ILGF1(antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-MILGF1abGP      $235.00/50ug

cat#RDI-MILGF1abGP1     $375.00/100ug

Rabbit anti-mouse ILGF1:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-MILGF1abrPB     $250.00/25ug

cat#RDI-MILGF1abrPB1   $375.00/50ug

 


DATA SHEET: Mouse anti-Human Insulin Like Growth Factor I (II*)

Price: $594.00/vial  cat#RDI-ILGF12abm

Package Size: 200 micrograms lyophilized 308ul of 0.1M   Tris-glycine pH7.4, 0.15M NaCl, 0.1M EDTA. 0.2micron filtered. no preservatives or carrier added) Reconstitute with 500ul sterile water (yielding a 400ug/ml stock solution)

Species: mouse IgG1 Protein G purified

Antigen: purified human IGF-I from human plasma

Specificity: specific for IGF-I of human, rat and mouse origin (**approx 40% cross with ILGF II), some cross with chicken IGF-I & 2. UPDATED CROSS REACTIVITY Study see Endocrinology, Oct 1997

Activity: Neutralization: 10-20 micrograms/ml to inhibit the stimulatory effect of 10 nanograms IGF-I/ml in cultures of chicken embryo fibroblasts.(neutralization can be hindered by stearic effects of rat IGF-1 binding protein.

Western blot: 0.5-1 micrograms/ml to detect 100ng IGF-I/IGF-II under non reducing conditions. Recommend overnight 4 DEG C incubation with enhanced chemiluminescence detection

-MUST TITER FOR EACH APPLICATION

-Immunoprecipitation (approx 5ug for 100ng IGF-1 using Protien-G Agarose)

-Immunohistochemistry (approx 10ug/ml for paraffin embedded methanol:acetone 50:50 fixed skin sections)

Storage: Upon receipt, store at -20 DEG C or below. -After reconstitution, aliquot and store at - 20 DEG C. Avoid frequent freeze thaw cycles.

ref: -Van Wyk, J.J. et al, Endocrinology Oct 1997

      -Russell, et al, Proc. Natl. Acad Sci 81:2389-2392(1984)

     -Gillespie, G.Y. et al, Meth. Enz. 146:207   (1987)

     -Van Wyk, J.J. et al, Human Growth Hormone, 585-599. Plenum Publishing Corp, NY (1986)

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.



RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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