rev:     January 12, 2005

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Cytokines, Growth Factors & Chemokines:ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


IL-1 B  (INTERLEUKIN 1 BETA)

(request Interleukin & Growth Factor Receptor Data Pack for more antibodies and spec sheets)

-see below for anti-human/rat/mouse/rabbit reactive antibodies

-For Elispot/bulk Elisa also see the Pelipairs(tm)


anti-human IL-1B (antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-IL1BabrP1     $375.00/100ug  (rabbit polyclonal) - DISCONTINUED

cat#RDI-IL1BabGP1  $375.00/100ug   (Goat polyclonal)

anti-human IL-1B:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-IL1BabrPB   $250.00/25ug  (rabbit)

cat#RDI-IL1BabGPB1  $495.00/50ug  (Goat)

Mouse anti-human IL-IL1B  500ug  cat#RDI-IL1BabmXP   $375.00  Bulk on request

anti-murine IL-1B (antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-mIL1BabrP     $250.00/50ug  (rabbit)

cat#RDI-mIL1BabGP1  $375.00/100ug  (Goat)

anti-murine IL-1B:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-mIL1BabrPB    $250.00/25ug  (rabbit)

cat#RDI-mIL1BabGPB1  $375.00/50ug (Goat)

Rabbit anti-RAT IL-1B (antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-RTIL1BabrP    $250.00/50ug

cat#RDI-RTIL1BabrP1  $375.00/100ug

Rabbit anti-RAT IL-1B:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-RTIL1BabrPB    $212.00/25ug

cat#RDI-RTIL1BabrPB1  $300.00/50ug


DATA SHEET: Rabbit anti-HUMAN IL-1 BETA


Catalog#: RDI-IL1Babr    $500.00/vial

LOT#


Package Size: 1.0 milligram purified IgG in 1.0ml PBS Buffer, pH 7.4. Prepared from neat rabbit serum by ammonium sulphate precipitation. Sterile filtered, NO PRESERVATIVES OR CARRIER PROTEINS ADDED. Endotoxin 1.256EU/mg by LAL.


Species: Rabbit IgG


Antigen: recombinant human IL-1B, 17.5kDa


Activity: Reacts with human IL-1 Beta (natural and recombinant forms).


Application -Neutralization:approx 20ug/ml will neutralize 100% of 9 pg/ml recombinant human IL1B. 1 pg/ml of human IL1B is equivalent to 1 unit of activity in this assay.
                 -Western blotting: use 10ug/ml with 100ng cytokine per lane.

                 -immunohistochemistry: start at 1:5 dilution evaluating against a positive control.
-All titers must be detemined in assay

Storage: Store at -20 Deg C upon receipt. Avoid   repeated freeze thaw cycles. A carrier   protein (BSA) or serum should be added to   diluted antibody to maintain the protein   concentration at 1mg/ml or greater. This   reduces antibody loss by blocking non   specific binding to container surfaces.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


ALSO Available, monocloanl anti-human IL-2B (3 clones)

rabbit anti-RAT IL-1 alpha cat#RDI-RTIL1Aabr $500.00

rabbit anti-RAT IL-1 BETA cat#RDI-RTIL1Babr $500.00


Monoclonal mouse anti-human interleukin-1, beta


CAT#: RDI-TRK41L12-6E10,   or   RDI-TRK41L12-11E5,  or  RDI-TRK41L12-5C10


PRICE: $375.00/mg or    $344.00/mg 2 or more


Clone: 6E10,   11E5,    5C10

These clones have been derived from hybridization of X63.Ag8.653 myeloma cells with spleen cells of a Balb/c mice immunized with recombinant human in terleukin-1,beta.


Reactivity: MoAb react with three different and spares determinants on IL-1 beta molecule.MoAb do not inhibit   the biological activity of IL-1.


Mouse isotype: IgG2b for clone 11E5

                        IgG2a for clone 6E10

                           IgM for clone 5C10


Application: Detection of IL-1 beta in two-site immunoassay.We recommend to use mixture of MoAb 6E10 and 11E5 for coating and MoAb 5C10 for labeling. Sensitivity of   such assay is 20 IU of IL-1 biological activity.


Detection of IL-1 beta in immunohistochemistry.

FOR IN VITRO RESEARCH USE ONLY


Rabbit anti-Rat Interleukin 1 Beta

Catalog#: # RDI-RTIL1Babr    $500.00/vial


LOT# see actual batch sheet


Package Size: 1.0 milligram purified Sterile filtered  (no carrier or preservative)


Species: Rabbit IgG


Activity: Reacts with RAT IL-1B (natural and recombinant forms). There is no cross reactivity with rat IL-1A, or IL-1A or IL1-B from mouse, rabbit or human origin.


Application -Neutralization: a dilution of 1:10 will partially neutralize LAF activity activity on murine C3H/Hej thymocytes.

                  -Western blotting: 1:10 to 1:50 dilution

                  -immunoprecipitation:1:10 to 1:100 can be used as the primary capture antibody followed by anti-rabbit IgG or                     a similar quantity can be bound to Protein A.

                   -RIA/ ELISA


Storage: Store at -20 Deg C. Avoid repeated freeze thaw cycles. For dilution, we recommend sterile PBS or media. A carrier protein (BSA) or serum should be added to diluted antibody to maintain the protein concentration at 1mg/ml or greater. This reduces antibody loss by blocking non specific binding to container surfaces.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.



RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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