rev:    December 27, 2004

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Cytokines, Growth Factors & Chemokines:ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


INTERFERON GAMMA

-also see our ELISA and compact kit  against human IFN-G


-ELISA against rat or mouse Interferon gamma

-also antibodies against interferon receptors

cat#RDI-M2533clb   Human IFN-gamma   Elispot kits $500.00


MOUSE MONOCLONAL ANTIBODY AGAINST HUMAN INTERFERON GAMMA (MMHG-1)


CAT# RDI-PB2150   $375.00/vial  


Quantity Shipped: 1 vial of 500 ug in 500 ul.


Concentration: 1 mg/ml.


Buffer: Phosphate buffered saline (PBS) with 0.1% BSA


Isotype: IgG1

Bioactivity: Binds to and neutralized human interferon gamma.


Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-gamma) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37, 755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387- 394]. In this antiviral assays for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-g provided by the National Institutes of Health [see Pestka, S. (1986)"Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material if prepared specifically for effective neutralization of Hu-IFN-G.


Usage: Western blot; immunoprecipitation;   immunohistochemistry; ELISA.

Shipping Temperature: Dry ice

Physical State of Product During Shipping: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -70'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.

-For In Vitro Research Use Only-


MOUSE MONOCLONAL ANTIBODY AGAINST HUMAN INTERFERON GAMMA (MMHG-2)

non-neutralizing


CAT# RDI-PB21550  $375.00/vial  


Quantity Shipped: 100 ug in 0.184 ml


Concentration: 0.545 mg/ml


Buffer: Phosphate buffered saline (PBS) with 0.1% BSA


Isotype: IgG1

Bioactivity: binds to but does not neutralize human interferon gamma.


Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-a) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37, 755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assays for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-a provided by the National Institutes of Health [see Pestka, S. (1986)"Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material binds Hu-IFN-y well, but does not neutralize Hu-IFN-G


Usage: Western blot; immunoprecipitation; immunohistochemistry; ELISA.

Shipping Temperature: Dry ice

Physical State of Product During Shipping: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -20'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.


RABBIT POLYCLONAL ANTIBODY AGAINST HUMAN INTERFERON GAMMA


CAT#: RDI-PB31500  $312.00/vial  


Quantity Shipped: 1 vial of 2 x 10(4) units in 0.1 ml.


Bioactivity: 2 x 10(5) neutralization units/ml


Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon gamma (Hu-IFN-G) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37, 755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assays for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-y provided by the National Institutes of Health [see Pestka, S. (1986)"Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material if prepared specifically for effective neutralization of Hu-IFN-G


Usage: Western blot; immunoprecipitation; immunohistochemistry; ELISA.

Shipping Temperature: Dry ice

Physical State of Product During Shipping: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -20'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.


-For In Vitro Research Use Only-


Rabbit anti-human IFN-Gamma (antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-IFNGabrP    $250.00/50ug

cat#RDI-IFNGabrP1   $375.00/100ug

Rabbit anti-human IFN-Gamma:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-IFNGabrPB      $250.00/25ug

cat#RDI-IFNGabrPB1    $375.00/50ug

Rabbit anti-mouse IFN-Gamma(antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-MIFNGabrP     $250.00/50ug

cat#RDI-MIFNGabrP1   $375.00/100ug

Rabbit anti-mouse IFN-Gamma:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-MIFNGabrPB     $250.00/25ug

cat#RDI-MIFNGabrPB1   $375.00/50ug

Goat anti-RAT IFN-Gamma(antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-RTIFNGabGP    $250.00/50ug

cat#RDI-RTIFNGabGP1   $375.00/100ug

Goat anti-RAT IFN-Gamma:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-RTIFNGabGPB      $250.00/25ug

cat#RDI-RTIFNGabGPB1    $375.00/50ug


Matched pairs (includes coating antibody, biotin labeled detection antibody and a calibrated standard) sufficient for up to 1000 wells

human IFN-Gamma cat#RDI-IFNG-KT       $656.00

mouse IFN-Gamma cat#RDI-MIFNG-KT   $656.00

rat IFN-Gamma       cat#RDI-RTIFNG-KT  $656.00


RABBIT anti-HUMAN INTERFERON GAMMA (neutralization)

Catalog#: RDI-IFNGabr   $438.00/vial

Package Size: 1 mg purified IgG (via ammonium sulfate   precipitation) in 1.0ml of PBS buffer, pH 7.4. NO preservative or carrier protein added. Sterile filtered.


Species: rabbit

Activity: Reacts specifically with human natural and recombinant interferon gamma. approx 20ug/ml will neutralize 4ng/ml of recombinant human interferon gamma.


Storage: Store at -20 DEG C immediatley upon arrival. Aliquot and avoid frequent freeze thaw cycles.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

It is the responsibility of the user to comply with all local/stateand Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.


DATA SHEET: Mouse anti-HUMAN INTERFERON GAMMA (Coating)

Catalog#: RDI-IFNGabm-C $562.00/vial

Package Size: 500 ug purified IgG (via Protein A) in 0.5 ml of PBS buffer. NO preservative or   carrier protein added. Sterile filtered.

Clone: NIM42

Species: mouse IgG1k

Activity: Reacts specifically with human natural and recombinant interferon gamma.

-IN western blot, use approx 5-10ug/ml.

-IN Elisa use as a coating antibody. Detecting ab=RDI-IFNGabm-D (suggest using Biotin conjugate, cat#RDI-IFNGabm-BT)

-neutralization:

Storage: Store at -20 DEG C immediatley upon arrival. Aliquot and avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not   for use in or on humans or animals or   for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.

also available with azide (non sterile) Bulk Quotes on Request

cat#RDI-IFNGabm-42 clone NIB42  $469.00/0.5mg

see also clones B27, clones 4S.B3


DATA SHEET: Mouse anti-HUMAN INTERFERON GAMMA:Biotin Conjugated   (Detecting)

Catalog#: RDI-IFNGabm-BT $562.00/vial

Package Size: 500 ug Biotin conjugated purified IgG in 0.5 ml of PBS buffer.

Species: mouse IgG1k (from ascites)

clone: 4S.B3

Immunogen: partially purified human FN-gamma from supernatants of human PBMC stimulated with Staph A.

Activity: Reacts specifically with human natural and recombinant interferon gamma.

-IN Elisa use as a DETECTING antibody. For matching coating ab=ab=RDI-IFNGabm-C

Storage: Store at 4-8 DEG C

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.


DATA SHEET: Mouse anti-HUMAN INTERFERON GAMMA (histochemistry)

Catalog#: RDI-IFNGabm-H $500.00/vial

LOT# see sheet with shipment

Package Size: 250 ug purified IgG (via Protein A) in 0.25 ml of PBS buffer. NO preservative or carrier protein added. Sterile filtered.

Species: mouse IgG2a

Activity: Reacts specifically with human natural and recombinant interferon gamma. No reactivity with human Interferon alpha or beta as determined in ELISA.

USES: -Immunocytochemistry: frozen cryostat sections were acetone fixed and incubated with this antibody over a broad titration range of from 5-50ug/ml. Must titer for each application.

-IN Elisa

-Neutralization: 1ng ab will neutralize approx 100% of anti-viral activity of 1 Unit of human IFN-G in a wish cell cytopathic effect assay using vesicular stomatitis virus. Pre-incubate antibody with IFN-G one hour at 37 DEG C prior to addition to cell line.

Dilution: Recommend diluting with 0.1-1% carrier (BSA or equivalent).

Storage: Store at -20 DEG C immediately upon arrival.

Aliquot and avoid frequent freeze thaw cycles. Antibody stable 6 months or longer frozen, undiluted or one month at 4 DEG C. Diluted material should be made fresh daily.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.



DATA SHEET: Rat anti-Mouse INTERFERON GAMMA    (Detecting/Neutralizing)

Catalog#: RDI-MIFNGabRT-DX      $594.00/vial

BULK QUOTES ON REQUEST

Package Size: 500 ug purified IgG (via Protein A)   in 0.5 ml of PBS buffer. NO preservative or carrier protein added. Sterile filtered.

Species: rat IgG1

Clone: XMG1.2

Activity: Reacts specifically with murine IFN-gamma.

              -IN western blot, use approx 1-5ug/ml, 2-3 hour RT or overnight 4 DEg C with chemiluminescence detection to detect < 100ng/lane under reducing conditions,

             -IN Elisa use the biotin labeled form as detecting antibody. Coating ab=RDI-MIFNGabrt-D

            -Neutralizing:
            -flow cytomtery (using FITC or PE conjugated form)

Storage: Store at 4 DEG C immediatley upon arrival. Aliquot and avoid frequent warming cycles. Handle under asceptic conditions

ref: J. Exp med 166:1229-1244

       Immunol Rev 127:5-24

       J. Immunol Meth 166:201-414

      J. Immunol Meth 173:219-228

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.

also available:Biotin labeled cat#RDI-MIFNGabrt-BT $562.00/0.5mg

                         FITC labeled cat#RDI-MIFNGabrt-FT $438.00/100ug

see also clone R4-6A2


DATA SHEET: Rat anti-Mouse INTERFERON GAMMA   (Capture/Neutralizing)

Catalog#: RDI-MIFNGabRT-CX    $594.00/vial

BULK QUOTES ON REQUEST

Package Size: 500 ug purified IgG (via Protein A) in 0.5 ml of PBS buffer. NO preservative or carrier protein added. Sterile filtered.

Species: rat IgG1

Clone: R4-6A2

Activity: Reacts specifically with murine IFN-gamma.

               -IN western blot, use approx 1-5ug/ml, 2-3 hour RT or overnight 4 DEg C with chemiluminescence detection to detect < 100ng/lane under reducing conditions,

              -IN Elisa use as the coating ab, pair with the biotin labeled dteecting ab cat#RDI- MIFNGabrt-BT $562.00/0.5mg

             -Neutralizing:

Storage: Store at 4 DEG C immediately upon arrival. Aliquot and avoid frequent warming cycles. Handle under asceptic conditions

ref: J. Exp Med 159:1560-1565

      J Exp Med 171:115-127

      Infect Immun 58:3225-3232

     J. Immunoassay 14:129-148

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.

also available with azide:cat#RDI-MIFNGabrtC $469.00/0.5mg

see also clone XMG1.2


RAT MONOCLONAL ANTIBODY AGAINST MOUSE INTERFERON GAMMA (RMMG-1)

CAT# RDI-PB22500  $375.00

Quantity Shipped: 1 vial of 500 ug in 0.197 ml.

Concentration: 2.54 mg/ml.

Buffer: Phosphate buffered saline (PBS)

Isotype: IgG1k

clone: RMMG-1

Bioactivity: Binds to mouse interferon gamma with high affinity (>10(9) M-1). Neutralizes mouse interferon gamma

Assay Used to Measure Bioactivity: Binding was determined to mouse interferon gamma (mouse IFN-G) by the method of Scatchard [Scatchard,G. (1949) Ann. N.Y. Acad.Sci. 51, 660-672].

Usage: Western blot; immunoprecipitation;   immunohistochemistry; ELISA.

Shipping Temperature: cool packs

Physical State of Product During Shipping: cold

Special Conditions/Comments: After receipt, this product should be kept at -20'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in sep- arate tubes so that freezing and thawing is kept to a minimum.

-For In Vitro Research Use Only-


RAT MONOCLONAL ANTIBODY AGAINST MOUSE INTERFERON GAMMA RECEPTOR CHAIN

1 (RMMGR-1) non-neutralizing.

CAT# RDI-PB22580    $375.00/vial

Quantity Shipped: 1 vial of 50 ug in 0.1ml wiht 0.1% BSA

Concentration: 0.5 mg/ml.

Buffer: Phosphate buffered saline (PBS)

Isotype: To be determined.

Bioactivity: Binds to but does not neurtralize mouse interferon gamma receptor.

Usage: Western blot; immunoprecipitation; immunohistochemistry; flow cytometry.

Shipping Temperature: cold pack

Physical State of Product During Shipping: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -20'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.

-For In Vitro Research Use Only-


MOUSE MONOCLONAL ANTIBODY AGAINST RAT Interferon Gamma (Neutralizing)

cat#RDI-PB23515 $469.00/500ug vial

Clone: DB-14

Quantity Shipped: 1 vial of 500ug

Concentration: 3 mg/ml

Buffer: phosphate buffered saline (PBS) and 0.1% BSA

Antigen: rat interferon gamma

Isotype: IgG2a

Bioactivity: binds to and neutralizes both rat and mouse interferon gamma; does not bind to human interferon gamma

Bioactivity: Binding was determined by the method of Van der Meide

Usage: western blot, immunoprecipitation, immunohistochemistry, ELISA.

Shipping Information

Shipping: Ice Packs

Physical State: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -20 C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.

This Product Is For Research Use Only. It Is Not For Human Use.


MOUSE MONOCLONAL ANTIBODY AGAINST RAT Interferon Gamma   (Neutralizing)

cat# RDI-PB23510 $469.00/500ug vial

Clone: DB-13

Quantity Shipped: 1 vial of 500ug

Concentration: 2 mg/ml (see batch sheet with shipment)

Buffer: phosphate buffered saline (PBS) and 0.1% BSA

Antigen: rat interferon gamma

Isotype: IgG1

Bioactivity: binds to and neutralizes rat interferon gamma; does not bind to mouse or human interferon gamma

Bioactivity: Binding was determined by the method of Van der Meide, 1986 gen Vitrol. 67, 1059.

Usage: western blot, immunoprecipitation, immunohistochemistry, ELISA.

Shipping Information

Shipping: Ice Packs

Physical State: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -20 C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.

This Product Is For Research Use Only. It Is Not For Human Use.


MOUSE MONOCLONAL ANTIBODY AGAINST RAT Interferon Gamma   (Neutralizing)

cat# RDI-PB23505 $469.00/500ug vial

Clone: DB-12

Quantity Shipped: 1 vial of 500ug

Concentration: 1 mg/ml (see batch sheet with shipment)

Buffer: phosphate buffered saline (PBS) and 0.1% BSA

Antigen: rat interferon gamma

Isotype: IgG1

Bioactivity: binds to and neutralizes rat interferon gamma; does not bind to mouse or human interferon gamma

Bioactivity: Binding was determined by the method of Van der Meide, 1986 gen Vitrol. 67, 1059.

Usage: western blot, immunoprecipitation, immunohistochemistry, ELISA

Shipping Information

Shipping: Ice Packs

Physical State: Frozen

Special Conditions/Comments: After receipt, this product should bekept at -20 C or below for retention of full activity. When thawing,the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.

This Product Is For Research Use Only. It Is Not For Human Use.


MOUSE MONOCLONAL ANTIBODY AGAINST RAT Interferon Gamma   (Neutralizing)

cat# RDI-PB23500 $469.00/500ug vial

Clone: DB-1

Quantity Shipped: 1 vial of 500ug

Concentration: 1 mg/ml (see batch sheet with shipment)

Buffer: phosphate buffered saline (PBS) and 0.1% BSA

Antigen: rat interferon gamma

Isotype: IgG1|

Bioactivity: binds to and neutralizesboth rat and mouse interferon gamma; does not bind to human interferon gamma

Bioactivity: Binding was determined by the method of Van der Meide, 1986 gen Vitrol. 67, 1059.

Usage: western blot, immunoprecipitation, immunohistochemistry, ELISA.

Shipping Information

Shipping: Ice Packs

Physical State: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -20 C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.

This Product Is For Research Use Only. It Is Not For Human Use.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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