rev:    May 8, 2007

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Cytokines, Growth Factors & Chemokines:ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


INTERFERON ALPHA

-see also kits for human Interferon Alpha Elisa kit

-also antibodies against interferon gamma receptor & anti-murine IFN-A  

-click here for a chart indicating the reactivity of some of our  anti-human interferon alpha subtype antibodies-


MOUSE MONOCLONAL ANTIBODY AGAINST HUMAN INTERFERON ALPHA (MMHA-11):FITC Conjugated

CAT# RDI-PB211123   $375.00/vial

Quantity Shipped: 1 vial of 50 tests.

Lot#

Concentration: 300ug in 0.15ml (2mg/ml)

F:P 5

Buffer: Phosphate buffered saline (PBS) with 0.1% BSA

Immunogen: Human leukocyte interferon

Bioactivity: Binds to and neutralizes human interfer- on alpha (in non-conjugated form)

Isotype: IgG1 k

Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-a) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37, 755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assays for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-a provided by the National Institutes of Health [see Pestka, S. (1986)"Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23].

Usage: Immunocytochemistry; Facs (titrate from 10ug/ml, 1-5ug/million cells)

Special Conditions/Comments: After receipt, this product should be kept at 2-8'C. Protect from light, Do Not Freeze. or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that warming is kept to a minimum.

-For In Vitro Research Use Only-

Not for use in preparation of any commerical kit


Monoclonal anti-human Interferon Alpha

cat#RDI-TRK4IF13    $375.00/1mg    $312.00/mg 2-5mg    $250.00/mg 6-20mg  INQUIRE FOR BULK


Clone: C10F5


Isotype: IgG1 kappa


Purification: Protein A Sepharose

Specificity: Recognizes common epitopes of alpha--A,-F,-D, -iL, -P   human interferon subtypes. Kd=2.4X 10 -10M

Application: -Detection in two site immunoassay of recombinant interferon alpha (subtypes -A,-F,-D,I1,-P). -Detection in two site immunoassay of natural interferon (assay sensitivity is 5 IU/ml). Recomend to use a rabbit polyclonal anti-interferon alpha A as a second antibody. -Affinity purification of recombinant interferons of   alpha-A,-F,-D subtypes.


PRESENTATION: Each monoclonal is delivered in a sealed vial at a   concentration of 1 milligram of murine monoclonal antibody dissolved in 1ml of phosphate buffered saline containing 0.1% sodium azide. We recommend that each laboratory determine an optimum working titer for use in its particular application.

Storage: Purified monoclonal antibodies are generally stable for period at 4 0C. However repeated warming to room temperature and re-cooling may result in loss of activity and hence working titre.

FOR IN VITRO RESEARCH USE ONLY. NOT FOR USE IN OR ON HUMANS OR ANIMALS-

NOT FOR DIAGNOSTICS. Not responsible for any Patent infringements with use or derivation of these products.


MOUSE MONOCLONAL ANTIBODY AGAINST HUMAN INTERFERON ALPHA (MMHA-2)

CAT# RDI-PB21100  $500.00/vial  $469.00/vial 3+

Quantity : 500ug

Concentration: 5mg/ml.

Buffer: Phosphate buffered saline (PBS) with 0.1% BSA

Isotype: IgG1 k

Antigen: human leucocyte interferon

Bioactivity: neutralizes human interferon alpha; binds to human interferon alpha with high affinity (=10(9) M-(1))

Assay Used to Measure Bioactivity: One neutralization unit is the amount ofantiserum required to neutralize one unit of human interferon alpha (Hu-IFN-a) to a 50% endpoint. Interferon was titrated with the use of thecytopathic effect inhibition assay as described [Rubinstein, S.,Familletti,P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37,755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenientand Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods inEnzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394]. Inthis antiviral assays for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-a provided by the National Institutes of Health [see Pestka, S. (1986)"Interferon Standards and General Abbreviations, in Methods in  Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material if prepared specifically for effective neutralization of Hu-IFN-a.

Usage: Western blot (approx 1:500);

             immunoprecipitation;

            immunohistochemistry; ELISA.

Shipping Temperature: ice pack

Physical State of Product During Shipping: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -70'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in sep- arate tubes so that freezing and thawing is kept to a minimum.  -For In Vitro Research Use Only-

Not for use in any commerical kit


cat#RDI-PB21100  $500.00/500ug  $469.00/vial 3+  Bulk quotes on request

cat#RDI-PB21101  $312.00/50ug  

cat#RDI-PB21105  $312.00/50ug

cat#RDI-PB21110  $312.00/50ug  

cat#RDI-PB21112  $312.00/50ug  

cat#RDI-PB21125  $312.00/50ug  

cat#RDI-PB21127  $312.00/50ug  

cat#RDI-PB21129  $312.00/50ug  

cat#RDI-PB21154  $312.00/50ug

cat#RDI-PB31100  $312.00/vial  

cat#RDI-PB31101  $312.00/vial  

cat#RDI-PB31130  $312.00/vial 

-click here for a chart indicating the reactivity of some of our  anti-human interferon alpha subtype antibodies-


SHEEP POLYCLONAL ANTIBODY AGAINST HUMAN INTERFERON ALPHA A


CAT# RDI-PB31100   $312.00/vial  

Description: Sheep Anti-Human Interferon Alpha A (SPHA-1).


Quantity Shipped: 1 vial of 1 x 10(5) units in 0.1 ml.


Bioactivity: 1 x 10(6) neutralization units/ml.er-

Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-a) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37, 755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assays for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-a provided by the National Institutes of Health [see Pestka, S. (1986)"Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material if prepared specifically for effective neutralization of Hu-IFN-a.


Usage: Western blot (1:500 dilution); immuno precipitation; immunohistochemistry; ELISA.

Shipping Temperature: Dry ice

Physical State of Product During Shipping: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -20'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.


-For In Vitro Research Use Only-


RABBIT POLYCLONAL ANTIBODY AGAINST HUMAN INTERFERON ALPHA A

-Neutralizing

CAT# RDI-PB31101   $344.00/vial  


Description: Rabbit Anti-Human Interferon Alpha A


Quantity Shipped: 1 vial of 2 x 10(4) units in 0.02 ml.


Bioactivity: 1 x 10(6) neutralization units/ml.


Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-a) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37, 755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assays for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-a provided by the National Institutes of Health [see Pestka, S. (1986)"Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material if prepared specifically for effective neutralization of Hu-IFN-a.


Usage: Western blot (1:500 dilution); immuno precipitation; immunohistochemistry; ELISA.


Shipping Temperature: Dry ice


Physical State of Product During Shipping: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -20'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.

-For In Vitro Research Use Only-


MOUSE MONOCLONAL ANTIBODY AGAINST HUMAN INTERFERON ALPHA D (MMHA-6)


CAT# RDI-PB21125   $312.00/vial  


Description: Mouse Anti-Human Interferon Alpha D


Quantity Shipped: 1 vial of 50 ug in 33 ul.


Concentration: 1.52 mg/ml.


Buffer: Phosphate buffered saline (PBS)


Isotype: IgG1 k

Bioactivity: Binds to and neutralizes human inter feron alpha.


Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-a) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37, 755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assays for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-a provided by the National Institutes of Health [see Pestka, S. (1986)"Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material if prepared specifically for effective neutralization of Hu-IFN-a.


Usage: Western blot; immunoprecipitation; immunohistochemistry; ELISA.


Shipping Temperature: Dry ice

Physical State of Product During Shipping: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -20'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.

-For In Vitro Research Use Only-


RABBIT POLYCLONAL ANTIBODY AGAINST HUMAN INTERFERON ALPHA F


CAT# RDI-PB31130  $312.00/vial  


Description: Rabbit Anti-Human Interferon Alpha F.

Quantity Shipped: 1 vial of 2 x 10(4) units in 0.125 ml.

Bioactivity: 1.6 x 10(5) neutralization units/ml.


Assay Used to Measure Bioactivity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-a) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S.,Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol.37, 755-758; Familletti,P.C., Rubinstein,S., and Pestka, S. (1981)" A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S.Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assays for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-a provided by the National Institutes of Health [see Pestka, S. (1986)"Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material if prepared specifically for effective neutralization of Hu-IFN-a.


Usage: Western blot; immunoprecipitation;   immunohistochemistry; ELISA.


Shipping Temperature: Dry ice


Physical State of Product During Shipping: Frozen

Special Conditions/Comments: After receipt, this product should be kept at -20'C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.


-For In Vitro Research Use Only-


RDI Div of Fitzgerald Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

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