rev:      October 17, 2004

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Cytokines, Growth Factors & Chemokines:ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


GM-CSF (Granulocyte Macrophage Colony Stimulating Factor)
Rabbit anti-human GM-CSF(antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-GMCSFabrP       $250.00/50ug

cat#RDI-GMCSFabrP1     $375.00/100ug

Rabbit anti-human GM-CSF:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-GMCSFabrPB       $250.00/25ug

cat#RDI-GMCSFabrPB1     $375.00/50ug

Rabbit anti-mouse GM-CSF(antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-MGMCSFabrP       $250.00/50ug

cat#RDI-MGMCSFabrP1      $375.00/100ug

Rabbit anti-mouse GM-CSF:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-MGMCSFabrPB      $250.00/25ug

cat#RDI-MGMCSFabrPB1    $375.00/50ug


Sample DATA SHEET:   SHEEP anti-HUMAN GM-CSF

Catalog#: RDI-GMSCFabs   $562.00/vial

LOT# see data sheet with shipment

Package Size: 1.0 milligram purified IgG

Species: sheep serum

Activity: Reacts with human GM-CSF (natural and recombinant forms). There is no cross reactivity with human IL-1A or B, Il-2, Il-4 ,Il-6 or Il-8.

Application -Neutralization: a dilution of 1:10-1:50 will neutralize human GM-CSF proliferation activity on the acute monocytic leukemia cell line AML-193.

-Western blotting: 1:10 to 1:50 dilution

-immunoprecipitation:1:10 to 1:100 can be used as the primary capture antibody followed by anti-rabbit IgG or a similar quantity can be bound to Protein A. -RIA/ ELISA

Storage: Store at -20 Deg C. Avoid repeated freeze thaw cycles. For dilution, we recommend sterile PBS or media. A carrier protein (BSA) or serum should be added to diluted antibody to maintain the protein concentration at 1mg/ml or greater. This reduces antibody loss by blocking non specific binding to container surfaces.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Sample DATA SHEET: Rat anti-HUMAN GM-CSF:    Capture ELISA AB/ELISPOT Capture

Catalog#: RDI-GMCSFabRC   $594.00/vial

Package Size: 500 micrograms purified IgG2a in 0.5ml (1mg/ml) PBS (no carrier, no preservative). 0. 2micron filtered. endootoxin <0.01ng/ug

Species: rat IgG2a

Clone: BVD2-23B6

Purification: Protein G from TCS

Activity: Reacts with human GM-CSF.

Application -ELISA:capture antibody 5-10ug/ml (see code RDI-GMCSFabRD-BT for matching detecting antibody, clone BVD2-23B6). Use with streptavidin-HRP/TMB detection system for maximum sensitivity.

                   -neutralization: preincubate antibody and ligand 60minutes), ED50 approx 8ng/ml using 200pg/ml human GM-CSF

                  -western blot:1-5ug/ml

                  -immunoprecipitation

Storage: Store at 4 Deg C under aseptic conditions. Do NOt FREEZE.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.

ALSo available with 0.09% sodium azide (no carrier but not 0.2micron filtered)

cat#RDI-GMCSFabrt-B6 $531.00/0.5mg Bulk on request

Note: The design of an ELISA protocol requires considerable work and skill. For small projects, we strongly recommend using preconfigured kits.


Sample DATA SHEET: Rat anti-HUMAN GM-CSF:Biotin labelled   DETECTING ELISA AB/ELISPOT

 Catalog#: RDI-GMCSFabRD-BT   $594.00/vial

Package Size: 500 micrograms in 1ml (0.5mg/ml) 10mM phosphate buffer pH7.2 with 150mM NaCl and 00.09% sodium azide.

Species: rat IgG2a

Clone: BVD2-21C11

Activity: Reacts with human GM-CSF.

Application -ELISA:detecting antibody 0.5-2ug/ml (see code RDI-GMCSFabRC for matching coating antibody, clone BVD2-23B6). Use with streptavidin-HRP/TMB detection system for maximum sensitivity.

Storage: Store at 4 Deg C. Do NOt FREEZE.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.

Note: The design of an ELISA protocol requires considerable work and skill. For small projects, we strongly recommend using preconfigured kits.


Sample DATA SHEET: Rat anti-HUMAN GM-CSF:   DETECTING ELISA AB/ELISPOT/Histochem

Catalog#: RDI-GMSCFabRD $  $250.00/0.1mg

LOT# see data sheet with shipm ent

Package Size: 100 micrograms purified IgG2a in 1ml PBS with 0.09% NaN3. -see batch sheet   for batch specific information.

Species: rat IgG2a

Clone: BVD2-21C11

Antigen: E. Coli expressed rec human GM-CSF

Activity: Reacts with human GM-CSF and rhesus M.

Application -ELISA/ELISPOT:detecting antibody 5-10ug/ml

                 -IMUNOHISTOCHEMISTRY/IFA

              -flow cytometry

             -western blot (reducing)

ref: -Eur J. Immunol. 24:1808-1815.

-Immunology 83:16-24

-Litton M., et al, Human Cytokine

Protocols, eds Debets and

Savelkoul, Human Press, in press

-Ped. Res. 34:213-216

Storage: Store at 4 Deg C. Do NOt FREEZE.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.


Sample DATA SHEET: Rat anti-Mouse GM-CSF:Biotin labeled   Detecting AB***

Catalog#: RDI-MGMSCFabD  $562.00/vial

LOT# see data sheet with shipment

Package Size: 500 micrograms biotin conjugated antibody in 1ml with 0.1% NaN3

Species: rat IgG1k

Clone: MP1-31G6

Immunogen: yeast derived recombinant mouse GM-CSF

Activity: Reacts with murine GM-CSF as the detecting antibody when paired with cat#RDI-MGMSCFABM-C as the coating antibody

Application ELISA:detecting antibody 0.1-1ug/ml (see code RDI-MGMCSFabC for matching coating antibody).

-Western blotting: 1-5ug/ml under non reducing conditions (detects approx 100ng/lane)

-Neutralization: (in purified form)

Reference: -J. Immunol Meth 166:201-214

                  -Cell Immunol 129:228-240

                  -J. Invest Dermatol 97:10-14

                 -Immunol Rev 127:5-24

Storage: Store at 4 Deg C. Avoid repeated freeze   thaw cycles.

Precautions: For In vitro research Use Only. Not   for use in or on humans or animals or   for diagnostics.


Sample DATA SHEET:   Rat anti-MOUSE GM-CSF

Catalog#: RDI-MGMSCFabC    $531.00/vial

LOT# see data sheet with shipment

Package Size: 500 micrograms purified antibody   in 500ul PBS with 0.1% NaN3

also available:azide free               cat#RDI-MGMCSFCXP    $688.00/0.5mg

                                FITC labeled cat#RDI-MGMSCFCFT    $469.00/100ug

                                   PE labeled cat#RDI-MGMCSFCPE     $500.00/100ug

Species: Rat IgG2a

Clone: MP1-22E9

Antigen: yeast expressed rec mouse GM-CSF

Activity: Reacts with mouse GM-CSF in Elisa (as coating ab when paired with cat#RDI-MGMCSFabD), neutralization (using azide free material) and immunoflourescence/flow cytometry (recommend using FITC or PE labeled material

-inquire)

Application -ELISA:Coating antibody 5-10ug/ml (see code RDI-MGMCSFabD for matching detecting antibody).-

-Neutralization:

-flow:

Storage: Store at 4 Deg C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.



RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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