rev: April 28, 2002

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


Description: Recombinant Human Erythropoietin (EPO) HIGH PURITY

cat# RDI-EPO-HPAG   (old cat#RDI-PB11965)

Price: $440.00/50ug ( 6,000 units)*

          $3750.00/500ug ( 60,000 units)*

          $5000.00/1mg (120,000 units)*

           Larger sizes quoted on request

Lot#: see spec sheet with shipment

Concentration: 390ug/ml ( 50ug=128ul, 500ug=1.28ml)

Specific Activity:* 120,000 U/mg as measured by Normocyth-aemic mice (see batch sheet for batch specific information)

Packaging: sterile filtered liquid formulation. The protein was frozen after extensive dialysis in 0.65mg sodium citrate, 4.1mg sodium chloride and 55ug citric acid. Endotoxin <0.1ng/ug (1EU/ug) of rhuEPO

Derivation: produced in CHO cells as a single polypeptide chain containing 165 amino acids and having a predicted molecular mass of 21,00 daltons and apparent glycosylated molecular mass of 30,400 daltons. Purified by a proprietary chromatographic technique. amino acid composition in total agreement with the expected amino acid composition of native human EPO

Solubility: very soluble in water and most aqueous buffers. Recommend diluting in sterile buffers containing suitable carrier protein as 1-3% BSA.

Purity: > 98% as determined by RP-HPLC, anion- exchange FPLC and analysis by reducing and non-reducing SDS-PAGE Silver stained. <1% dimer and aggregates as determined by silver stained SDS-PAGE gel analysis.

Storage: Stable at 4 DEG C for up to 4 week. Recommend making working aliquots and store at -70 DEG C. At first thaw, Before opening, the tube should be centrifuged briefly at 4'C to concentrate the contents on the bottom of the tube. Dilute only in buffers containing protein such as 1% to 3% serum albumin.

This Product Is For In Vitro Research Use Only-not for use in or on humans or animals.



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