rev:    September 7, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


DATA SHEET:   RECOMBINANT Human Soluble CD4

cat# RDI-CYT304X    $5625.00/mg   $5000/mg 2+

       RDI-CYT304S     $688.00/100ug

Bulk quotes on request

Description: Recombinant Human sCD4 produced in Baculovirus is a single, glycosylated polypeptide chain containing 365 amino acids and having a non glycosilated molecular mass of 40,000 Dalton. As a result of glycosilation the sCD4 migrates as a 46,000 dalton protein on standard SDS-PAGE. Detected By Western Imunno Blots anti cd4 monoclonal antibody. Recombinant soluble CD4 is purified by proprietary chromatographic techniques.

CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MHC (Major Histocompatibility Complex) Class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as HIV receptors, binding directly to the envelope protein gp120 on HIV. Thymocytes which are CD4 (+)and CD8 ( - )produce factors such as IL-4 , IL-5 ,IL-10 and IFN-gamma

Appearance: Sterile Filtered White lyophilized (freeze-dried powder.

Source: Baculovirus

Endotoxin: Less than 0.1 ng/µg (IEU/µg) of sCD4

Formulation: The protein was lyophilized from a solution of 0.5X PBS with no additives

Purity: Greater than 95.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Anion-exchange FPLC.

(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis

Solubility: It is recommended to reconstitute the lyophilized material in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rsCD4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rSCD4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze-thaw cycles.

Biological Activity: This recombinant Human Soluble CD-4 is fully biologically active when compared to standard. Immune reactive in ELISA using monoclonal anti CD4   in gpl2O capture ELISA assays.

Protein content: Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm

2. Analysis by RP-HPLC, using a standard solution of sCD4 as a Reference Standard.

For In vitro research Use Only. Not for use in or on humans or animals .  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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