rev:     August 20, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:Recombinant Human IGFBP-1   (Insulin Like Growth Factor Binding Protein 1) BULK

cat# RDI-CYT299X   $6250.00/mg

       RDI-CYT299S   $250.00/20ug        Bulk quotes on request

Description: IGFBP-1 is one of the six homologous proteins that specifically bind insulin-like growth factors and modulate their mitogenic and metabolic actions. The function of this protein is not completely defined. However, several studies demonstrate that it inhibits IGF binding to cell surface receptors and thereby inhibits IGF-mediated mitogenic and cell metabolic actionsInsulin Like Growth Factor Binding Protein modulate IGF activities by increasing their plasma half lives and by inhibiting or promoting the interactions of IGF with receptors on certain target cells. In addition these binding proteins provide a reservoir for IGF in pericellular spaces. Some IGF-BPs also have stimulating effects in vitro and some may inhibit the growth of cells

Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Source: E.Coli

Endotoxin: Less than 0.1 ng/µg (IEU/µg) of IGFBP1

Formulation: The protein was lyophilized from a solution of 10mM HCL with no additives

Purity: Greater than 95.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Anion-exchange FPLC.

(c) Analysis by reducing and non-reducing SDS-PAGE

Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained

SDS-PAGE gel analysis

Solubility: It is recommended to reconstitute the lyophilized material in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rIGFBP1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rIGFBP1 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze-thaw cycles.

Biological Activity: IGFBP-1 is fully biologically active when compared to standards. Inhibition of IGF-I stimulated proliferation of NUC-1 cells: IC50 ~ 100 ng/ml Inhibition of serum-induced stimulation of DNA synthesis in chick embryo fibroblast primary cultures: IC50 (1% serum) ~300 ng/ml.

Protein content: Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm

2. Analysis by RP-HPLC, using a standard solution of IGFBP-1 as a Reference Standard.

see also IGFBP2, IGFBP-3, recombinant human ILGF1, rechuILGF2,and more...

Precautions: For In vitro research Use Only. Not for use continued on back in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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