rev: July 28, 2000

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


Recombinant Human CD137-huIgG (4-1BB) fusion Protein

Background: Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival and induces monocyte activation.

cat# RDI-CD137FU-AG    Price: $531.00/25ug

package: 25 micrograms (preservative free) in 5ul (0.5mg/ml) 50mM sodium phosphate pH7.5, 100mM Potassium Chloride, 150mM NaCl.Product was filtered through 0.1um and vialed under aseptic conditions. Tighten Cap and centrifuge 1 minute at 1000 rpm to concentrate material in vial prior to use.

Cell Line: CHO

Mw: a soluble 55kd fusion protein consisting of the extracellular (186aa) domain of human CD137 fused to human IgG1 fc.

Activity: This human CD137 Ig fusion protein blocks binding of an anti-human CD137 o activated CEM human tunor cells and also binds to CD137 Ligand on Raji cells.

-can be used in histochemistry (frozen sections) and flow cytometry

Storage: 2-5 DEG C (DO NOT FREEZE) aliquot and store in working aliquots.

ref: Blood 87:2839-2845 (1996)


Assay: 500K human Raji cells were washed and incubated 45 minutes on ice with 80ul of CD137-huIg at 5ug/ml. Cells were washed twice and incubated with 50ul of secondary antibody (Goat anti-human IgG:FITC) after which they were washed three times, fixed and analyzed by Facs. Cells stained positive with a mean shift of 0.48 log10 fluorescent units when compared to a buffer control at a similar concentration. Binding was blocked when reagent was preincubated 30 minutes with CD137L-muCD8 at a concentration of 50ug/ml.

For In Vitro Research Use Only.

also available:

conjugated to Biotin cat#RDI-CD137FUBT    $625.00/25ug

                       FITC cat#RDI-CD137FUFT     $750.00/50 tests

                          PE  cat#RDI-CD137FUPE     $938.00/50 tests


Recombinant Human CD137L-muCD8 (4-1BB LIGAND) fusion Protein

Background: Human CD137L (4-1BB Ligand) is a type II transmembrane protein constitutively expressed by monocytes, B cells and neuroblastoma cells . Binding of CD137 (4-1BB) to CD137L induces monocyte activation.

cat# RDI-CD137LFU-AG     Price: $531.00/25ug

package: 25 micrograms (preservative free) in 50ul (0.5mg/ml) 50mM sodium phosphate pH7.5, 100mM Potassium Chloride, 150mM NaCl.Product was filtered through 0.1um and vialed under aseptic conditions. Tighten Cap and centrifuge 1 minute at 1000 rpm to concentrate material in vial prior to use.

Cell Line: Murine myeloma cell line P363Ag8.653

Mw: a soluble molecule consisting of the extracellular domain (184aa) of human CD137L fused to the extracellular domain (167aa) of murine CD8 alpha.

Activity: This human CD137-Ligand fusion protein binds to CD137 and blocks binding of anti-CVD137 monoclonal antibody.
-can be used in histochemistry (frozen sections) and flow cytometry

Storage: 2-5 DEG C (DO NOT FREEZE) aliquot and store in working aliquots.

ref: J Immunol 160:2488-2494 (1998)

Assay:Cultured human CEM cells were stimulated 1 day in the   presence of PMA(10ug/ml) and ionomycin (1uM). 500K cells were   washed and incubated for 45 minutes on ice with 80ul of CD137L- muCD8 at 10ug/ml. Cells were washed twice and incubated with anti-mouse CD8A/RPE after which they were washed three times, fixed and analyzed by Facs. Cells stained positive with a mean shift of 1.02 log10 fluorescent units when compared to a buffer control at a similar concentration. Binding was blocked when reagent was preincubated 30 minutes with CD137-huIg at a concentration of 50ug/ml.

For In Vitro Research Use Only.

also available: conjugated to

Biotin     cat#RDI-CD137LFUBT     $625.00/25ug

FITC      cat#RDI-CD137LFUFT     $750.00/50 tests

PE          cat#RDI-CD137LFUPE     $938.00/50 tests


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