rev:   March 5, 2005

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Cytokines, Growth Factors & Chemokines:  

(Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies.


BONE MORPHOGENETIC PROTEINS & RECEPTORS

-see also BMP antibodies    (see BMP-2 & BMP-14)


  Recombinant Human BMP-2

Description: Human Bone Morphogenetic Protein-2 (BMP-2) is a disulfide-bonded homodimeric protein with an apparent molecular weight of 26 kDa. BMP-2 regulates similiarly to its nearest homologue BMP-4 diverse fundamental processes during embryonic development: BMP-2 and other BMP proteins have great potential for medical therapeutic applications, in particular because they allow or at least accelerate the ossification of extensive bone lesions. BMP-2 lacks the natural N-terminus which results in a 15-20 fold increase of specific activity. BMP-2 is a heparin binding protein.

Source:                              E. coli

Molecular Weight:            26 kDa

Specific activity:                5 x 10 4 units/mg

Purity:                               > 95% by SDS-PAGE and visualised by silver stain

Endotoxin level:               < 0.1 ng per mg of BMP-2

Stabilizer:                         none

Buffer:                              none

Formulation:                     lyophilized

Biological Activity: The ED50 for the proteoglycan synthesis in embryonal chicken limb bud cells has been determined to be in the range of 20 to 30 ng/ml..

Reconstitution: The lyophilized BMP-2 is soluble in distilled water and most aqueous buffers at concentrations greater than 1 mg/ml when the pH is below 6.0. Above pH 6.0 the solubility is low, but could be increased by addition of 1 M NaCl or 30% 2-propanol or with 10mM Sodium Citrate buffer pH3.5

Stability: Lyophilised samples are stable for greater than six months at -20°C to -70°C. Reconstituted BMP-2 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!

Usage: BMP-2 is offered for research use. Not for drug use. Not for human use!

Catalogue number: RDI-200-002      $535.00           Size: 25 µg

                          Bulk quotes on request

Literature: [Wozney et al., Science 242:1528, 1988; Ruppert et al., Eur J Biochem 237 :295, 1996]


DATA SHEET:recombinant Human BMP-2

-BMPs (bone morphogenetic proteins) belong to the TGF-beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF-beta superfamily are disulfide-linked dimmers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate promote and regulate bone development, growth, remodeling and repair. However, it is now clear that in addition to their role in bone and cartilage morphogenesis, BMPs are also involved in prenatal development and postnatal growth of eye, heart, kidney, skin and other tissues. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis. It is also expressed in a variety of tissues such as lung, spleen, brain, liver, prostate ovary and small intestine. This recombinant human BMP-2 is a 26 kDa homodimeric protein consisting of two 115 amino acid polypeptide chains.

Catalog#: RDI-1202

Package Size: 10 micrograms $235.00   $219.00/2or more

                    MINI-PACK cat#RDI-1202p $94.00/2ug        see bulk form below

Supplied: sterile filtered and the the protein was lyophilized from 10mM Sodium Citrate pH3.5( no additives)

Source: E. Coli

Purity: >98% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/ug).

Reconstitution: The lyophilized We recommend a quick spin followed by reconstitution in 10mM Sodium Phosphate pH3.5 OR diluting into water containing BSA (50µg of BSA per 1µg of protein) to a concentration of 0.1-1.0 mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at -4 DEG C for up to 1 week or at -20 DEG C for future use.

Note:the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration >10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 Deg C. Reconstituted human BMP-2 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 as determined by the cytolysis of MC3T3-E1 cells is < 50ng/ml.

AA Sequence:

MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF

PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV

LKNYQDMVVE GCGCR

For In vitro research Use Only. Not for use in or on humans or animals .  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Recombinant Human Bone Morphogenetic Protein -2   (rHuBMP-2) BULK

cat# RDI-1202-261X     $6250.00/1mg   $5625/mg 2-4  Bulk quotes on request

        RDI-1202-261X5   $3750.00/0.5mg

Description: Recombinant Human BMP-2 produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26018 Dalton. The rHuBMP-2 is purified by proprietary chromatographic techniques.

Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Source: E.Coli

Endotoxin: Less than 0.1 ng/µg (IEU/µg) of BMP-2 .

Formulation: The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=3.5.

Purity: Greater than 95.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Anion-exchange FPLC.

(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Ala-Lys-His.

Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Solubility: It is recommended to reconstitute the lyophilized rHuBMP-2 in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rHuBMP-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution rHuBMP-2 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze-thaw cycles.

Biological Activity: BMP-2 is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of MC3T3-E1 cells is less then 50 ng/ml.

Protein content: Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of BMP-2 as a Reference Standard.

For In vitro research Use Only. Not for use in or on humans or animals .  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.



-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.
RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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