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ANTI-RAT CYTOCHROME P450

& Related Antibodies  

(anti-RAT and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-RAT CYP450 Antibodies (inquire for others)


DATA SHEET: Rabbit anti-Cytochrome P450 Side Chain   Cleavage Enzyme

Background:P450scc enzyme is the rate limiting enzyme controlling steroidogenesis. P450scc is responsible for the conversion of cholesterol to pregnenolone. The enzyme is expressed in all tissues capable of steroidogenesis, including the ovary, adrenal, testis, placenta and glial cells.

Catalog#: RDI-P450SCCabr  $438.00/vial

Batch#

Package Size: 100ul neat sera (no preservative)

Species: rabbit serum

Antigen: synthetic antigen of amino acids 421-441 of rat cytochrome P450 side chain cleavage (P450scc) enzyme)

Specificity: -Antibody reacts with rat, mouse, bovine, hamster, porcine and rabbit.

Titer: -Optimize for each method

            -western blot: 1:1000

             -immunohistochemistry on frozen or Bouin's  fixed paraffin embedded sections: 1:200 using Streptavidin:Biotin detection systems

ref: Molecular and Cellular Endocrinology (1991) 79:13-20.

Storage: Store at -20 DEG C or lower. AVOID FREQUENT FREEZE THAW CYCLES. Do not freeze diluted antibody.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


see also rabbit anti-rat/mouse P450 side chain cleavage  (aa509-526 cat#RDI-P450SCBabr    $375.00/100ul


DATA SHEET: Rabbit anti-Human/Rat Cytochrome P450   REDUCTASE

Catalog#: RDI-P450REDabr $438.00/vial    

Batch#

Package Size: 100ul neat sera (no preservative)

Species: rabbit serum

Antigen: synthetic peptide

Specificity: -Antibody reacts with human and rat cytochrome P450 reductase in hepatic microsomal fractions. Also binds to this enzyme in several other species.

Titer: -Optimize for each method

             -western blot:

related ref: -Federation Proc (1987) 46:2563-2566

                 -Adv. Enzymol. (1987) 60:35-87

                 -DNA and Cell Biology (1993) 12:1-51

                -Biochemical Pharmacology (1995) 49:39-47

Storage: Store at -20 DEG C or lower. AVOID FREQUENT FREEZE THAW CYCLES. Do not freeze diluted antibody.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Rabbit Anti-Rat Cytochrome P450 Enzymes CYP2B1 and CYP2B2     and human CYP2B6

Cat#: RDI-CYP2B12abr   $438.00/vial


Quantity: 100 ul neat rabbit sera

Specificity: Reacts with rat cytochrome P450 enzymes CYP2B1 and CYP2B2 in hepatic microsomal fraction. Also reacts with human CYP2B6. No cross-reactivity with other cytochrome P450 enzymes.

Immunogen: Synthetic peptide

Applications: Western blot: CYP2B1 and CYP2B2 can be separated electrophoretically. Immunohistochemistry on frozen and formaldehyde treated sections. Optimal working dilutions must be determined by end user.

Storage/Handling: Maintain at -20'C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Gen ref: -Federation Proc. (1987) 46:2563-2566.

              -Adv.Enzymol. (1987) 60:35-87.

               -DNA and Cell Biology (1993) 12:1-51.

               -Biochemical Pharmacology (1995) 49:39-47.

For Research Use Only; Not for Use as a Diagnostic.

Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 ul or less, we recommend gentlytapping the vial on a hard surface or briefly centrifuging the  vial in a tabletop centrifuge to dislodge and liquid in the container's cap.


see also mouse anti-rat CYP2B1/2 (also sees 2A1 weakly)

cat#RDI-RTCYP2B12abm $438.00/vial (approx 10 blots)

see also mouse anti-rat CYP2B1 (specific)

cat#RDI-RTCYP2B1abm $438.00/vial (approx 10 blots)

no cross with rat 2B2 or 2A1


Rabbit Anti-Cytochrome P450 Enzymes CYP2C8/9/19 and rat CYP2C12

Cat#: RDI-CYP2C8912abr    $438.00/vial

Quantity: 100 ul neat rabbit sera

Specificity: Reacts with human CYP2C8, CYP2C9 and CYP2C19 and RAT CYP2C12 in hepatic microsomal fraction.

Immunogen: Synthetic peptide

Applications: Western blot: CYP2C8/9/19 and rat CYP2C12 migrate at approx 56kda on SDS-PAGE. Recommend full size blots for best CYP450 separation.

-Immunohistochemistry on frozen and formaldehyde treated sections.

-Optimal working dilutions must be determined by end user.

Storage/Handling: Maintain at -20'C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Gen ref: -Federation Proc. (1987) 46:2563-2566.

             -Adv.Enzymol. (1987) 60:35-87.

            -DNA and Cell Biology (1993) 12:1-51.

           -Biochemical Pharmacology (1995) 49:39-47.

For Research Use Only; Not for Use as a Diagnostic.

Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial.For products with volumes of 200 ul or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the  vial in a tabletop centrifuge to dislodge and liquid in the container's cap.


Mouse Anti-Rat Cytochrome P450 Enzymes CYP2C11

Cat#:  RDI-RTCYP2C11Abm    $438.00/vial

Lot#:

Quantity: 200 ul mouse IgG fraction

Specificity: Reacts with rat cytochrome P450 enzymes 2C11 in hepatic microsomal fraction.

Immunogen: Synthetic peptide

Applications: -Western blot: 1:500 with chemiluminescence detection (4 DEG C overnight), load 10ug rat microsomes or equivalent per lane. -must titer to optimize for each application

Storage/Handling: Stable for several weeks at 4 DEG C, several months at -20 DEG C and almost idenfinetly at -80 DEG C. Avoid repeated freeze/thaw cycles.

General ref: J. Immunol Mehtods 100, 123-130 (1987)

For Research Use Only; Not for Use as a Diagnostic.


Sheep Anti-Rat/Human/Mouse Cytochrome P450 Enzymes CYP1A2

Cat#: RDI-RTCYP1A2abS    $440.00/vial

Quantity: 100 ul neat sheep sera

Specificity: Reacts with rat cytochrome P450 enzymes 1A2 in hepatic microsomal fraction. No cross-reactivity with other cytochrome P450 enzymes including CYP1A1.

Immunogen: Synthetic peptide

Applications: Western blot:

Immunohistochemistry on frozen and formaldehyde treated sections. Optimal working dilutions must be determined by end user.

Reactivity: rat, human, mouse

Storage/Handling: Maintain at -20'C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Gen ref: -Federation Proc. (1987) 46:2563-2566.

              -Adv.Enzymol. (1987) 60:35-87.

               -DNA and Cell Biology (1993) 12:1-51.

              -Biochemical Pharmacology (1995) 49:39-47.

For Research Use Only; Not for Use as a Diagnostic.

Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial.For products with volumes of 200 ul or less, we recommend brieflycentrifuging the vial in a tabletop centrifuge to dislodge and liquid in the container's cap.


Rabbit Anti-Rat Cytochrome P450 Enzymes CYP1A1

Cat#: RDI-RTCYP1A1abr   $440.00/vial

Quantity: 100 ul neat rabbit sera

Specificity: Reacts with rat cytochrome P450 enzymes 1A1 in hepatic microsomal fraction. No cross-reactivity with other cytochrome P450 enzymes including CYP1A2

Immunogen: Synthetic peptide

Applications: Western blot:

Immunohistochemistry on frozen and formaldehyde treated sections. Optimal working dilutions must be determined by end user.

Storage/Handling: Maintain at -20'C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

For Research Use Only; Not for Use as a Diagnostic.

Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial.For products with volumes of 200 ul or less, we recommend brieflycentrifuging the vial in a tabletop centrifuge to dislodge and liquid in the container's cap.


Sheep Anti-Rat Cytochrome P450 Enzymes CYP1A1

Cat#: RDI-RTCYP1A1abS    $438.00/vial

Quantity: 100 ul neat sheep sera

Specificity: Reacts with rat cytochrome P450 enzymes 1A1 in hepatic microsomal fraction.

Immunogen: Synthetic peptide

Applications: Western blot: 1:4000

Storage/Handling: Maintain at -20'C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

For Research Use Only; Not for Use as a Diagnostic.

Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial.For products with volumes of 200 ul or less, we recommend briefly centrifuging the vial in a tabletop centrifuge to dislodge and liquid in the container's cap.


Monoclonal Anti-Rat Cytochrome P450 (1A1)

Product cat#  RDI-RTP4501A1abm     $438.00

Presentation: 100ul (approx 0.5mg/ml) Monoclonal Mouse IgG.

Specificity: Identification of the polycyclic aromatic induced 1A1 by immunoassay or Western blots. This antibody does not cross react with 1A2.

Applications: Immunoassay or Western blots.

Contents: 100 µL per vial. Contains sufficient purified monoclonal mouse IgG for at least 10 Western blots.

Dilution: A dilution of 1/1000 for Western blots will yield a strong 1A1 band (59kDa) with 10 µg rat microsomes obtained following 3-methylcholanthrene (3-MC) (25mg/kg) treatment.

Storage: Store at 4°C. Do not freeze and thaw repeatedly. Refreezing the product may result in diminishment or loss of activity.

References: Thomas, P.E., et al. (1984) J. Biol. Chem. 259: 3890.

For Research Use Only


Rabbit Anti-Rat Cytochrome P450 Enzymes CYP3A1

Cat#: RDI-RTCYP3A1abr   $438.00/vial

Lot#:

Quantity: 100 ul neat rabbit sera

Specificity: Reacts with rat cytochrome P450 enzymes 3A1 in hepatic microsomal fraction.

Immunogen: Synthetic peptide

Applications: Western blot: 1:4000 with chemiluminescence detection (4 DEG C overnight)

Storage/Handling: Maintain at -20'C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

For Research Use Only; Not for Use as a Diagnostic.

Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial.For products with volumes of 200 ul or less, we recommend brieflycentrifuging the vial in a tabletop centrifuge to dislodge and liquid in the container's cap.


Rabbit Anti-Rat Cytochrome P450 Enzymes CYP3A2

Cat#: RDI-RTCYP3A2abr    $438.00/vial

Lot#:

Quantity: 100 ul neat rabbit sera

Specificity: Reacts with rat cytochrome P450 enzymes 3A2 in hepatic microsomal fraction.

Immunogen: Synthetic peptide

Applications: -Western blot: 1:4000 with chemiluminescence detection (4 DEG C overnight)

                        -histochemistry

                        -must titer to optimize for each application

Storage/Handling: Maintain at -20'C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

General ref: Biochemical Pharmacology (1995) 50:2047-2056

For Research Use Only; Not for Use as a Diagnostic.


Rabbit anti-Rat CYP2C11 POLYCLONAL ab

cat# RDI-RTCYP2C11abr   $438.00/vial

type: rabbit (new Zealand)

package: 100ul of rabbit serum diluted in PBS with 0.05% NaN3

antigen: synethetic peptides corresponding to the amino and carboxy terminus of rat CYP450 2C11

peptide A: N terminal D(49)-I-G-Q-S-I-K-K-F-S-K-V(60)

peptide B: C terminal Q(491)-R-A-D-S-L-S-S-H-L(500)

USE: -detects a major band at approx 50kDa by western blot Using MALE rat liver and brain. FEMALE rat liver microsomes do not express this enzyme (negative control). Also identified P50 2C11 in cat. approx dilution 1:1500 for WB when using chemiluminescence detection

-in histochemsitry, shows a perivascular staining when tested on male at brain samples (approx 1:100 dilution)

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.|

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Rabbit Anti-Rat Cytochrome P450 Enzymes CYP2D1

Cat#: RDI-RTCYP2D1abr     $438.00/vial

Quantity: 100 ul neat rabbit sera

Specificity: Reacts with rat cytochrome P450 enzymes 2D1 in hepatic microsomal fraction.

Immunogen: Synthetic peptide

Applications: -Western blot: with chemiluminescence detection (4 DEG C overnight)

                      -histochemistry

-must titer to optimize for each application

Storage/Handling: Maintain at -20'C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

General ref: Brain Research (in press)

For Research Use Only; Not for Use as a Diagnostic.

Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial.For products with volumes of 200 ul or less, we recommend briefly centrifuging the vial in a tabletop centrifuge to dislodge and liquid in the container's cap.

inquire for antibodies to rat: CYP1A1, CYP1A2, CYP2B1/2, CYP2C12, CYP2D1, CYP2D4, CYP2E1, CYP3A2, CYP4A1/2/3, CYP450 reductase, CYP 450 side chain cleavage and other against human


RABBIT ANTI-RAT CYTOCHROME P450 CYP2D4   POLYCLONAL ANTIBODY

CAT# RDI-RTCYP2D4abr   $438.00

LOT NUMBER:

QUANTITY: 100 µL neat sera (liquid with no preservatives)

SPECIFICITY: By immunohistochemistry the antibody reveals strong staining in rat brain, but not liver, consistant with CYP2D4.

IMMUNOGEN: Synthetic peptide

APPLICATIONS: Immunohistochemistry Optimal working dilutions must be determined by end user.

STORAGE: Maintain at -20

For Research Use Only

Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial.For products with volumes of 200 ul or less, we recommend brieflycentrifuging the vial in a tabletop centrifuge to dislodge and liquid in the container's cap.

inquire for antibodies to rat: CYP1A1, CYP1A2, CYP2B1/2, CUP2B1(specific), CYP2C12, CYP2D1, CYP2D4, CYP2E1, CYP3A2, CYP4A1/2/3, CYP450 reductase, CYP 450 side chain cleavage and other against human


RABBIT ANTI-HUMAN/RAT CYTOCHROME P450 ENZYME CYP2E1   POLYCLONAL ANTIBODY

cat# RDI-RTCYP2E1abr   $438.00

Presentation: 100ul diluted rabbit serum (no preservative)

IMMUNOGEN: Synthetic peptide

SPECIFICITY: Reacts with human and rat cytochrome P450 enzyme CYP2E1 in hepatic microsomal fractions. No cross-reactivity with other cytochrome P450 enzymes in these species.

APPLICATIONS: Western blot

                             Immunohistochemistry on formaldehyde treated and frozen sections.

                              Optimal working dilutions must be determined by end user.

STORAGE: Maintain at -20C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

RELATED

 REFERENCES:

Federation Proc. (1987) 46:2563-2566.

Adv. Enzymol. (1987) 60:35-87.

DNA and Cell Biology (1993) 12:1-51.

Biochemical Pharmacology (1995) 49:39-47.

For Research Use Only; Not for Use as a Diagnostic.

inquire for antibodies to rat: CYP1A1, CYP1A2, CYP2B1/2, CYP2C12, CYP2D1, CYP2D4, CYP2E1, CYP3A2, CYP4A1/2/3, CYP450 reductase, CYP 450 side chain cleavage and other against human CYp450's


RABBIT ANTI-RAT CYTOCHROME P450 CYP4A1/4A2/4A3

cat# RDI-RTCYP4A123abr  $438.00

Presentation: 100ul rabbit sera (no preservative) -at first use, tighten cap, centrifuge 1 minute 1000rpm to concentrate material in vial.

IMMUNOGEN: Synthetic peptide

SPECIFICITY: Binds specifically to cytochrome P450 CYP4A1, CYP4A2 and CYP4A3 in rat hepatic microsomal fraction.

APPLICATIONS: Western blot: approx 1:1000, band 55/58 kDa on reducing gels

                               Immunohistochemistry:4% PFA fixed, frozen tissue recommended.

                              -not tested with other species

                             Optimal working dilutions must be determined by end user.

STORAGE: Maintain at -20C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

For Research Use Only

Also available: sheep anti-rat CYP4A1/2/3 cat#RDI-CYP4A123abS  $438.00/100ul for blotting


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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