rev: January 16, 2000

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GLUTATHIONE TRANSFERASES (GST's)

RECOMBINANT    ENZYMES 

(see also our special line of   native  and  recombinant  cytochrome P450 enzymes)


Drug Metabolizing Enzymes -for in vitro research use only-

Glutathione Transferases (GST's) Recombinant Human


Product#                  Isoform              Quantity           Price**

RDI-P2175               A1-1                   100ug           $438.00

RDI-P2192               M1-1                  100ug           $438.00

RDI-P2177               P1-1                    100ug          $438.00

  *300.00/vial for 5 or more any combination

Applications:

In Vitro Drug Metabolism & Toxicology Research &  Studying Activation of Procarcinogens

Description

Glutathione transferases (GST's) catalyze the formation of  thioether conjugated between glutathione and reactive xenobiotic compounds. Their major biological function is believed to be defense against electrophilic chemical species, many of which are formed by cellular oxidative reactions catalyzed by cytochromes P450 and other oxidases.


Four structural classes of cytosolic GSTs have been identified in mammals based on primary structures: Alpha,Mu,Pi, and Theta. The enzymes exist as dimers of approximately 25 kDa subunits from a single structural class. RDI recombinant human GSTs are all over-produced in E. coli and purified by affinity chromatography. GST A1-1 is found in only a few tissues of the body, including kidneys, intestine, lung and liver; GST M1-1 is also restricted to a few tissues including the liver; GST P1-1 is abundant in most types of tumor cells, as well as being widely distributed throughout the body (except for its notable absence from the liver).The GST M1-1 is the type b allelic variant, which corresponds to GST y purified from human liver. The physical and catalytic properties of all three enzymes are indistinguishable from enzyme purified from human tissue.

Specifications:(see sheet with shipment)

Source: Recombinant E. coli

Concentration: 1-5 mg/ml in 50 mM Tris-HC1, pH 7.5,50 mM NaC1, 1mM NaC1, 1 mM DTT, 1mM EDTA,50%                                  glycerol.


Physical Purity: Greater than 95% as assessed by visualization of 10 mg of protein on a Coomassie-stained SDS gel.


Activity: Determined using spectrophotometric determination of 1- chloro-2,4-dinitro- benzene (CDNB) conjugation in 100 mM KPO4, pH 6.5. Minimum acceptable specific activities,in mmol/min/mg are 50 for GST A1- 1, 100 for M1-1, and 75 for P1-1.


Specificity: The different specificities of the three GST isozymes are demonstrated by determining the I50 values obtained using the inhibitor S- hexylglutathione in the standard CDNB assay: I50 (A1-1)< I50 (<M1-1) < 50 (P1-1).


References:

1. Mannervik, B. and Widersten,M. (1995) in Adv. in Drug Metab.in Man, Pacifici, G.M. & Fracchis, G.N., eds,European Commission, Luxemburg: 407-459

2. Widersten,M., Pearson,W.R.,Engstrom,A., Mannervik,B.(1991) Biochem, J. 276: 519-524.

3. Stenberg, G.Bjornestedt,R.,Mannervik,B.(1992) Protein Express- ion Purif. 3: 80-84

4. Kolm, R.H.,Stenberg,G., Widersten,M., Mannervik,B.,(1995) Protein Expression Purif. 6: 265-271.


see also our native and recombinant Cytochrome P450 enzymes and antibodies


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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