rev:  January 23, 2002

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ANTI-CYTOCHROME P450

& Related Antibodies  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-CYP2C9


Rabbit anti-human Cytochrome P450 CYP2C9


CAT# RDI-CYP2C9abr
Price: $440.00/1mg vial


Presentation: 1 milligram lyophilized
Anti-human CYP2C9 IgG is provided as a powder after lyophylization from 100 mM potassium phosphate buffer (pH7.4), 150 mM KCl, and 2.5 μM thimerosal (added as a preservative). The whole IgG fraction was purified from antiserum using caprylic acid/ammonium sulfate fractionation.
 
Specificity and Purity:
Specificity has been determined by Western blotting. Anti-human CYP2C9 IgG reacts primarily with CYP2C9 (56 kDa) but also recognizes CYP2C19 (50 kDa) in human liver microsomes; cross-reaction with CYP2C8 (52 kDa) is minimal. The antibody also cross-reacts with the homologous CYP2C proteins in rat, rabbit, and hamster liver microsomes. Specificity with whole liver homogenates or S-9 fractions has not been determined. Antibody purity has been established by SDS-PAGE run under denaturing conditions. Two protein bands with molecular weights of 50 kDa and 25 kDa can be visualized by Coomassie blue staining, which correspond to the heavy and light chains, respectively, of rabbit IgG.
 
Reconstitution of Lyophylized Product and Storage:
Prior to reconstitution, store product at 0-5°C. Reconstitute by adding 1 ml of PBS/50% glycerol to one vial
of lyophilized IgG (1 mg) and mix vial gently until powder dissolves. After reconstitution, the solution can
be stored at -20°C, as the presence of glycerol will prevent freeze/thaw cycles. Anti-CYP2C9 IgG solutions
without glycerol should also be stored at -20°C but seldom subjected to freeze/thaw.


 


Sample western blot data using the rabbit anti-CYP2C9 antibody Immunoreactivity of Anti-CYP2C9 IgG with human liver proteins (MW CYP2C9 = 56,000 daltons)

Lane A = UN504 microsomes (10 µg)

Lane B = Purified CYP2C9 (0.1 µg)

Lane C = UC9303 microsomes (10 µg)

Lane D = UC9406 microsomes (10 µg)


 



Anti-CYP2C9 IgG Reactivity with Human Liver Proteins
Lane 1 = UCSF003 microsomes (10 μg)
Lane 2 = Purified CYP2C19 (0.1 μg)
Lane 3 = Purified CYP2C8 (0.1 μg)
Lane 4 = Purified CYP2C9 (0.1 μg)

 
 

Use for Western Blotting:
For full size (15 x 15 cm) blots, add 100μg rabbit anti-human CYP2C9 IgG to 15-20 ml of blocking
solution (e.g., PBS containing 5% non-fat dry milk). This corresponds to 100 μl of the 1.0 mg IgG/ml
solution prepared as described above. The amount can be reduced with smaller size blots.
Incubate overnight at room temperature, and remove antibody solution. After washing blot with blocking
solution to remove unbound CYP2C9 antibody, incubate with an anti-rabbit IgG conjugate of choice (e.g,
anti-rabbit IgG-peroxidase, anti-rabbit IgG-alkaline phosphatase or anti-rabbit IgG-biotin), and develop
accordingly.


 



Precaution: For in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Not responsible for any patent infringements with the use or derivation of   these products.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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