rev:  May 8, 2007

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ANTI-CYTOCHROME P450

& Related Antibodies  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-CYP2C19


Rabbit anti-human Cytochrome P450 CYP2C19   NEW MONOSPECIFIC ANTIBODY



Rabbit anti-human Cytochrome P450 CYP2C19 NEW FORMULATION   MONOSPECIFIC


CAT# RDI-CYP2C19Mabr


 Price: $1000.00/1mg vial


Antiserum was developed in rabbits using purified recombinant human CYP2C19 as immunogen. The whole IgG fraction was purified from antiserum using caprylic acid/ammonium sulfate fractionation. Anti-human CYP2C19 IgG is provided as a powder lyophilized from 100 mM potassium phosphate buffer (pH 7.4), 150 mM KCl, and 2.5 µM thimerosal (added as a preservative).

- Specificity and Purity
Specificity has been determining by Western blotting. Anti-human CYP2C19 IgG reacts extensively with its immunogen as well as with CYP2C9 (56 kDa) in human liver microsomes; cross-reactivity with CYP2C8 (52 kDa) is much less, however. Reactivity of anti-human CYP2C19 with CYP2C proteins in liver microsomes from other animal species is unknown. Antibody purity has been established by denaturing SDS-PAGE. Two protein bands with molecular weights of 50 kDa and 25 kDa can be visualized by Coomassie blue staining, which correspond to the heavy and light chains, respectively, of rabbit IgG.

- Reconstitution of Lyophylized Product and Storage
Prior to reconstitution, store product at 0-5°C. Reconstitute by adding 0.5-1.0 ml of PBS/50% glycerol to one vial of lyophilized IgG (1 mg) and mix vial gently until powder dissolves. After reconstitution, the solution can be stored at -20°C as the presence of glycerol will prevent freeze/thaw cycles.

Reaction of Anti-CYP2C19 igG with human liver proteins
        1         2         3         4         5

Reaction of Anti-CYP2C19 IgG with human liver proteins
Lane 1 = UCSF003 Liver Mx (2C19+) 15 µg
Lane 2 = UC9408 Liver Mx (CYP2C19-) 15 µg
Lane 3 = Purified CYP2C9 0.1 µg
Lane 4 = Purified CYP2C8 0.1 µg
Lane 5 = Purified CYP2C19 0.1 µg

- Use for Western Blotting
1. For full size (15 x 15 cm) blots, add 150 µg rabbit anti-human CYP2C19 IgG to 15-20 ml of blocking solution (e.g., PBS containing 5% non-fat dry milk). This corresponds to 150 µl of the 1.0 mg IgG/ml solution prepared in 50% glycerol as described. The amount can be reduced with smaller size blots.
2. Incubate overnight at room temperature, and remove antibody solution (which can be stored at -20°C for re-use). After washing blot extensively with blocking solution to remove unbound CYP2C19 antibody, incubate with an anti-rabbit IgG conjugate of choice (e.g, anti-rabbit IgG peroxidase, anti-rabbit IgG-alkaline phosphatase or anti-rabbit IgG-biotin), and develop accordingly.

Inquire for:pure natural P450 enzymes


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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