rev:     January 5, 2005

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!


Recombinant Human DESMIN

cat#RDI-PRO62016      $690.00/250ug

cat#RDI-PRO62216      $406.00/100ug

Description                    Recombinant human DESMIN

Molecular Weight:         53,539.6 (calculated from sequence)

                                       55,000 (determined by SDS gel electrophoresis)

Source:                           E. Coli

Purity:                            Greater than 95% (determined by SDS gel electrophoresis ) see below

Application:                   -Protein standard in 1D and 2D SDS

                                       -Immunoassays

                                       -Immunization

Reconstitution:               100ug: When reconstituted with 70ul distilled water (final volume 100ul),

                                       250ug: when reconstituted with 175ul distilled water (final volume 250ul)

concentration of DESMIN is 1mg/ml in 30mM Tris/HCL pH 8, 9.5M urea, 2mM DTT, 2mM EDTA, 10mM methylammonium chloride

Storage:                         At 2-8 DEG C (lyophilized) Store -20 DEG C after reconstitituion Avoid frequent freeze thaw cycles.

-reconstitution to filaments is performed by mixing equimolar amounts of cytokeratins of Type 1 and type II at concentrations of approx 0.5mg/ml, both dissolved in 9.5M urea buffer (as per above formulation) Protofilaments and filament complexes are obtainined by dialyzing the resulting polypeptide stepwise to a concentration of 4M urea and then to low salt concentration (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCL, pH 7.4).

see J. Cell Biol 101, 1826-1841 (1995) and J. Mol. Biol 197, 237-255 (1987)


Strictly for in vitro research use only-not for use in or on humans or animals-not for diagnostics


Our product cat. no. RDI-PRO62016 (recombinant desmin) also contains low amounts (< 5%) of the 45 kD polypeptide, which is a proteolytic degradation product of desmin and represents the alpha-helical rod piece of the polypeptide. This 45 kD fragment cannot be eliminated totally by our purification methods. When reconstituting the lyophilized product the rod piece is readily soluble whereas the intact polypeptide takes more time to get resolubilized. Whenever the applicant is not investing sufficient time for resolubilization the more soluble 45 kD fragment becomes over-represented in the sample taken for e.g. SDS-PAGE and Western blot analysis. In total homogenates of tissue the 45 kD fragment is found after unsufficient measures against proteolytic degradation.

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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