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(for in vitro research use only)
RDI Division of Fitzgerald Industries Intl offers a wide line of antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!
Description Recombinant human DESMIN
Molecular Weight: 53,539.6 (calculated from sequence)
55,000 (determined by SDS gel electrophoresis)
Source: E. Coli
Purity: Greater than 95% (determined by SDS gel electrophoresis ) see below
Application: -Protein standard in 1D and 2D SDS
Reconstitution: 100ug: When reconstituted with 70ul distilled water (final volume 100ul),
250ug: when reconstituted with 175ul distilled water (final volume 250ul)
concentration of DESMIN is 1mg/ml in 30mM Tris/HCL pH 8, 9.5M urea, 2mM DTT, 2mM EDTA, 10mM methylammonium chloride
Storage: At 2-8 DEG C (lyophilized) Store -20 DEG C after reconstitituion Avoid frequent freeze thaw cycles.
-reconstitution to filaments is performed by mixing equimolar amounts of cytokeratins of Type 1 and type II at concentrations of approx 0.5mg/ml, both dissolved in 9.5M urea buffer (as per above formulation) Protofilaments and filament complexes are obtainined by dialyzing the resulting polypeptide stepwise to a concentration of 4M urea and then to low salt concentration (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCL, pH 7.4).
see J. Cell Biol 101, 1826-1841 (1995) and J. Mol. Biol 197, 237-255 (1987)
Strictly for in vitro research use only-not for use in or on humans or animals-not for diagnostics
RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266