rev: September 8, 1999

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!


PRODUCT DATA SHEET

STANDARD: FILAMIN

CAT# RDI-PRO62006     PRICE:$375.00

Molecular Weight 250 000

Source Chicken gizzard

Application Protein standard in 1D and 2D SDS- gel electrophoresis

                  Immunoassay

                 Antigen for immunisation

Purity Greater than 90%; determ. by SDS-PAGE

1)     filamin;                               ____

2)     myosin (a),                                        ____a

        B-galactosidase (b),                           ____b

        phosphorylase B (c),                          ____c

        BSA (d),                                           ____d

        ovalbumin (e)

                                                                 ____e

                                                          1        2


Amount 250ug (lyoph., salt balanced)


ORDER NO:RDI-PRO62006 (250ug)


Isoelectric point pHl 6.4


Reconstitution (250ug) when reconstituted with 250ul Laemmli buffer or 9 M urea (eg IEF- or NEPHGE buffer),sol.(final vol. 250ug) will additionally contain 20mM Tris/Acetate pH .6,20mM NaCl, 0.1mM EDTA, 2mM DTT,with a protein conc. of 1 mg/ml (according to Bradford protein determination).


Storage at 4Deg.C (lyoph.)    at -20Deg.C )sol.)


References

Feramisco J.R. and Burridge,K.: A rapid purification of alpha-actinin, filamin, and a 130,000 dalton protein from smooth muscle J. Biol Chem, 255, 1194 ff, (1980)


Davies,P.J.A.,Wallach,D.,Willingham,M.,Pestan, I.and Lewis, M.S.:Self-association of chicken gizzard filamin and heavy merofilamin,Biochem, 19, 365 ff, (1984)


For In Vitro Research Use Only


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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