rev: September 8, 1999

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!


PRODUCT DATA SHEET


STANDARD:CYTOKERATIN NO 18 (from bovine liver)


CAT# RDI-PRO62004   PRICE:$375.00

Molecular Weight 45 000


Source Bovine liver


Application :Protein standard in 1D and 2D SDS- gel electrophoresis

                   Immunoassay

                   Antigen for immunisation


Purity Greater than 98%; determ.by SDS-PAGE


1)  phosphorylase B(a),                  a ____

     BSA (b),                                   b ____

     ovalbumin (c),

     carbonic anhydrase (d);              c ____        ____

2)  cytokeratin no 18

                                                      d ____

                                                            1               2


Amount 250ug (lyoph.,salt balanced)


ORDER NO RDI-PRO62004   (250ug)


Isoelectric point pHI 5.4


Reconstitution (250ug) when reconstituted with 170ul H20 bidest,sol.(final vol. 250ul)will contain 30mM Tris/HCl pH 8.0,9M urea, 2mM DTT,with a protein conc. of 1mg/ml (according to protein dry weight).


Storage at 4 Deg.C (lyoph.)

REFERENCES: Franke, W.W., Denk, et al, Biochemical and immunological identification of cytokeratin proteins present in hepatocytes of mammalian liver tissue. Exp. Cell Res. 131, 299 ff (1981)

Quinlan, R.A. et al, Heterotypic tetramer (A2D2) complexes of non epidermal keratins isolated from cytoskeletons of rat hepatocytes and hepatoma cells, J. Mol. Bio. 178, 365 ff (1984)


For In Vitro Research Use Only,


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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