rev:September 8, 1999

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!


PRODUCT DATA SHEET


STANDARD: CYTOKERATIN NO 8

CAT# RDI-PRO62003     PRICE: $375.00

Molecular Weight 52 000

Source Bovine liver

Application Protein standard in 1D and 2D SDS- gel electrophoresis

                  Immunoassay

                  Antigen for immunisation


Purity Greater than 95%; determ.by SDS-PAGE


                                                               a _____

                                                               b _____

  1)     phosphorylase B(a),                                           ____

          BSA (b),                                        c _____

          ovalbumin (c),

          carbonic anhydrase (d);

2)       cytokeratin no 8                             d _____

                                                                     1                 2


Amount 250ug (lyoph.,salt balanced)


ORDER NO :RDI-PRO 62003 (250ug)


Isoelectric point pHl 6.4


Reconstitution (250ug) when reconstituted with 170ul H20 bidest,sol. (final vol. 250ul)will contain 30mM Tris/HCl pH 8.0, 9M urea, 2mM DTT,with a protein conc. of 1mg/ml (according to protein dry weight).

Storage at 4Deg.C (lyoph.)


References

Franke,W.W.,Denk,H.,Kalt,R. and Schmid,E.: Biochemical and immunological identification of cytokeratin proteins present in hepatocytes of mammalien liver tissue.Exp.Cell Res. 131,299 ff.(1981)


Quinlan,R.A.,Cohlberg,J.A.,Schiller,D.L.,Hatzfald,M.and Franke,W.W.: Heterotypic tetramer A202) complexes of non-epidermal keratins isolated from cytoskeletons of rat hepatocytes and hepatoma cells,J.Mol.Biol. 178,365 ff,(1984)


For In Vitro Research Use Only.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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