rev: July 27, 2002

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!


SAMPLE SPEC SHEET: HUMAN GH (GROWTH HORMONE)

-for in vitro research use only-not for internal or external administration to humans or animals


-hGH (human Growth Hormone) is produced in the anterior pituitary gland and regulates growth and metabolism of most human tissues.Natural growth hormone is 22K dalton and a 20K Dalton variant is present in human pituitary glands.

BATCH SPECIFIC SPEC SHEET: Human Growth Hormone-High Purity

cat: RDI-GH-HP

lot# $562.00/1mg    $250.00/100ug

Specific                            2.25IU/mg

Immunological Activity:

Reference Prep: 1st IS 80/505 WHO

Purity: >95% by SDS-Page

Level of Contaminants:    hTSH      0.006%       0.0006 IU/mg  ACS180

                                         hLH        0.004%       0.4IU/mg        Abbott IMX

                                       hFSH        0.005%       0.2IU/mg       Abbott IMX

                                       hPRL        0.6%           0.006mg/mg   Abbott IMX

Storage: -20 DEG C Upon Receipt

Form: Lyophilized powder, lyophilized from 50mM ammonium bicarbonate

Source: Human Pituitary glands

Reference assay: Human GH Nichols hGH assay

For IN VITRO Research or Further Manufacturing Use Only. NOT FOR IN VIVO USE IN HUMANS OR ANIMALS.

Serum from donors has been tested and found negative for hepatitis BSAg, HCV, HIV1, and HIV2 by currently approved FDA methods. However because no test method can offer complete assurance that HIV, HCV or other infectious agents is absent, this material should be handled at BioSafety Level 2 as recommended for any potentially infectious human serum or blood specimen in the CDC/NIH manula "Biosafety in Microbiological and Biomedical Laboratories," 1984.

FOR IN VITRO or FURTHER MANUFACTURING USE ONLY-NOT FOR USE IN DIAGNOSTICS.


Description: Recombinant Human Growth Hormone (rHuGH)   HIGH PURITY

cat# RDI-RGH-HPAG

Price: $440.00/1mg      $312.00/mg 10 or more   Larger sizes quoted on request

Lot#:

Concentration: 1 mg lyophilized

Specific Activity: 3 U/mg when compared to WHO reference standard

Packaging: sterile filtered prior to lyophilization. The protein was lyophilized from 5mg/ml stock after extensive dialysis against 1.7mg sodium phosphate buffer (0,1mg sodium phosphate monobasic and 1.6mg sodium phosphate dibasic) Endotoxin <0.1ng/ug (1EU/ug) of rhuGH

Derivation: produced in E Coli as a single, non glycosylated. polypeptide chain containing 192 amino acids and having a predicted molecular mass of 22,260 daltons Purified by a proprietary chromatographic technique. amino acid composition in total agreement with the expected amino acid composition of native human growth hormone

Solubility: very soluble in water and most aqueous buffers. Recommend diluting in sterile DI water followed by addition of suitable carrier to any diluted material as 1-3% BSA/HSA.

Purity: > 99% as determined by RP-HPLC, anion- exchange FPLC and analysis by reducing and non-reducing SDS-PAGE Silver stained. <1% dimer and aggregates as determined by silver stained SDS-PAGE gel analysis.

Protein content: A280 extinction coefficient 0.72 for 0.1% 1mg/ml solution.

Storage: Lyophilized material although stable at room temp for several weeks is best stored desiccated at -20 DEG C . After reconstitution, store at 4-8 DEG C for up to 1 month or make working aliquots and store -20 DEG C. Avoid frequent freeze thaw cycles.

This Product Is For In Vitro Research Use Only-not for use in or on  humans or animals.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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